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Engineering isoprenoid quinone production in yeast
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Kaur, Divjot, Alkhder, Duha, Corre, Christophe and Alberti, Fabrizio (2020) Engineering isoprenoid quinone production in yeast. ACS Synthetic Biology . doi:10.1021/acssynbio.0c00081 (In Press)
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Official URL: https://doi.org/10.1021/acssynbio.0c00081
Abstract
Isoprenoid quinones are bioactive molecules that include an isoprenoid chain and a quinone head. They are traditionally found to be involved in primary metabolism, where they act as electron transporters, but specialized isoprenoid quinones are also produced by all domains of life. Here, we report the engineering of a baker’s yeast strain, Saccharomyces cerevisiae EPYFA3, for the production of isoprenoid quinones. Our yeast strain was developed through overexpression of the shikimate pathway in a well-established recipient strain (S. cerevisiae EPY300) where the mevalonate pathway is overexpressed. As a proof of concept, our new host strain was used to overproduce the endogenous isoprenoid quinone coenzyme Q6, resulting in a nearly 3-fold production increase. EPYFA3 represents a valuable platform for the heterologous production of high value isoprenoid quinones. EPYFA3 will also facilitate the elucidation of isoprenoid quinone biosynthetic pathways.
| Item Type: | Journal Article | ||||||||||||
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| Subjects: | Q Science > QK Botany Q Science > QP Physiology |
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| Divisions: | Faculty of Science > Chemistry Faculty of Science > Life Sciences (2010- ) Faculty of Medicine > Warwick Medical School |
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| SWORD Depositor: | Library Publications Router | ||||||||||||
| Library of Congress Subject Headings (LCSH): | Isopentenoids, Ubiquinones, Yeast -- Genetic aspects, Saccharomyces cerevisiae -- Genetic aspects, Shikimic acid | ||||||||||||
| Journal or Publication Title: | ACS Synthetic Biology | ||||||||||||
| Publisher: | American Chemical Society | ||||||||||||
| ISSN: | 2161-5063 | ||||||||||||
| Official Date: | 20 August 2020 | ||||||||||||
| Dates: |
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| Date of first compliant deposit: | 1 September 2020 | ||||||||||||
| DOI: | 10.1021/acssynbio.0c00081 | ||||||||||||
| Status: | Peer Reviewed | ||||||||||||
| Publication Status: | In Press | ||||||||||||
| Publisher Statement: | “This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Synthetic Biology, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see [insert ACS Articles on Request author-directed link to Published Work, see http://pubs.acs.org/page/policy/articlesonrequest/index.html].” | ||||||||||||
| Access rights to Published version: | Restricted or Subscription Access | ||||||||||||
| RIOXX Funder/Project Grant: |
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