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Low DMSO cryopreservation of stem cells enabled by macromolecular cryoprotectants
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Murray, Kathryn, Tomás, Ruben M. F. and Gibson, Matthew I. (2020) Low DMSO cryopreservation of stem cells enabled by macromolecular cryoprotectants. ACS Applied Bio Materials, 3 (9). pp. 5627-5632. doi:10.1021/acsabm.0c00638 ISSN 2576-6422.
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Official URL: https://doi.org/10.1021/acsabm.0c00638
Abstract
Mesenchymal stromal (stem) cells have potential in regenerative medicine and modulating the immune system. To deliver any cell-based therapy to the patient, it must be cryopreserved, most commonly in DMSO, which impacts cell function and causes clinical side effects. Here we report the use of a synthetically scalable polyampholyte to rescue the cryopreservation of mesenchymal stromal cells in low [DMSO] cryopreservation. Flow cytometry showed retention of key markers of multipotency comparable to 10% (v/v) DMSO, and the cells could be differentiated, showing this polymer material can be used to improve, or replace, current cryopreservation strategies.
Item Type: | Journal Article | |||||||||||||||||||||
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Subjects: | Q Science > QD Chemistry Q Science > QH Natural history R Medicine > R Medicine (General) |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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SWORD Depositor: | Library Publications Router | |||||||||||||||||||||
Library of Congress Subject Headings (LCSH): | Cryopreservation of organs, tissues, etc. , Stem cells -- Cryopreservation , Biomedical materials , Polymers, Polyampholytes , Flow cytometry | |||||||||||||||||||||
Journal or Publication Title: | ACS Applied Bio Materials | |||||||||||||||||||||
Publisher: | American Chemical Society (ACS) | |||||||||||||||||||||
ISSN: | 2576-6422 | |||||||||||||||||||||
Official Date: | 21 September 2020 | |||||||||||||||||||||
Dates: |
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Volume: | 3 | |||||||||||||||||||||
Number: | 9 | |||||||||||||||||||||
Page Range: | pp. 5627-5632 | |||||||||||||||||||||
DOI: | 10.1021/acsabm.0c00638 | |||||||||||||||||||||
Status: | Peer Reviewed | |||||||||||||||||||||
Publication Status: | Published | |||||||||||||||||||||
Reuse Statement (publisher, data, author rights): | ** From Crossref via Jisc Publications Router ** History: epub 28-08-2020; issued 28-08-2020. | |||||||||||||||||||||
Access rights to Published version: | Open Access (Creative Commons) | |||||||||||||||||||||
Copyright Holders: | Copyright © 2020 American Chemical Society | |||||||||||||||||||||
Date of first compliant deposit: | 17 September 2020 | |||||||||||||||||||||
Date of first compliant Open Access: | 18 September 2020 | |||||||||||||||||||||
RIOXX Funder/Project Grant: |
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