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Low DMSO cryopreservation of stem cells enabled by macromolecular cryoprotectants
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Murray, Kathryn, Tomás, Ruben M. F. and Gibson, Matthew I. (2020) Low DMSO cryopreservation of stem cells enabled by macromolecular cryoprotectants. ACS Applied Bio Materials . doi:10.1021/acsabm.0c00638 (In Press)
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Official URL: https://doi.org/10.1021/acsabm.0c00638
Abstract
Mesenchymal stromal (stem) cells have potential in regenerative medicine and modulating the immune system. To deliver any cell-based therapy to the patient, it must be cryopreserved, most commonly in DMSO, which impacts cell function and causes clinical side effects. Here we report the use of a synthetically scalable polyampholyte to rescue the cryopreservation of mesenchymal stromal cells in low [DMSO] cryopreservation. Flow cytometry showed retention of key markers of multipotency comparable to 10% (v/v) DMSO, and the cells could be differentiated, showing this polymer material can be used to improve, or replace, current cryopreservation strategies.
| Item Type: | Journal Article | |||||||||||||||||||||
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| Subjects: | Q Science > QD Chemistry Q Science > QH Natural history R Medicine > R Medicine (General) |
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| Divisions: | Faculty of Science > Chemistry Faculty of Medicine > Warwick Medical School |
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| SWORD Depositor: | Library Publications Router | |||||||||||||||||||||
| Library of Congress Subject Headings (LCSH): | Cryopreservation of organs, tissues, etc. , Stem cells -- Cryopreservation , Biomedical materials , Polymers, Polyampholytes , Flow cytometry | |||||||||||||||||||||
| Journal or Publication Title: | ACS Applied Bio Materials | |||||||||||||||||||||
| Publisher: | American Chemical Society (ACS) | |||||||||||||||||||||
| ISSN: | 2576-6422 | |||||||||||||||||||||
| Official Date: | 28 August 2020 | |||||||||||||||||||||
| Dates: |
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| Date of first compliant deposit: | 17 September 2020 | |||||||||||||||||||||
| DOI: | 10.1021/acsabm.0c00638 | |||||||||||||||||||||
| Status: | Peer Reviewed | |||||||||||||||||||||
| Publication Status: | In Press | |||||||||||||||||||||
| Publisher Statement: | ** From Crossref via Jisc Publications Router ** History: epub 28-08-2020; issued 28-08-2020. | |||||||||||||||||||||
| Access rights to Published version: | Open Access | |||||||||||||||||||||
| Copyright Holders: | Copyright © 2020 American Chemical Society | |||||||||||||||||||||
| RIOXX Funder/Project Grant: |
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