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Low DMSO cryopreservation of stem cells enabled by macromolecular cryoprotectants

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Murray, Kathryn, Tomás, Ruben M. F. and Gibson, Matthew I. (2020) Low DMSO cryopreservation of stem cells enabled by macromolecular cryoprotectants. ACS Applied Bio Materials, 3 (9). pp. 5627-5632. doi:10.1021/acsabm.0c00638 ISSN 2576-6422.

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Official URL: https://doi.org/10.1021/acsabm.0c00638

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Abstract

Mesenchymal stromal (stem) cells have potential in regenerative medicine and modulating the immune system. To deliver any cell-based therapy to the patient, it must be cryopreserved, most commonly in DMSO, which impacts cell function and causes clinical side effects. Here we report the use of a synthetically scalable polyampholyte to rescue the cryopreservation of mesenchymal stromal cells in low [DMSO] cryopreservation. Flow cytometry showed retention of key markers of multipotency comparable to 10% (v/v) DMSO, and the cells could be differentiated, showing this polymer material can be used to improve, or replace, current cryopreservation strategies.

Item Type: Journal Article
Subjects: Q Science > QD Chemistry
Q Science > QH Natural history
R Medicine > R Medicine (General)
Divisions: Faculty of Science, Engineering and Medicine > Science > Chemistry
Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School
SWORD Depositor: Library Publications Router
Library of Congress Subject Headings (LCSH): Cryopreservation of organs, tissues, etc. , Stem cells -- Cryopreservation , Biomedical materials , Polymers, Polyampholytes , Flow cytometry
Journal or Publication Title: ACS Applied Bio Materials
Publisher: American Chemical Society (ACS)
ISSN: 2576-6422
Official Date: 21 September 2020
Dates:
DateEvent
21 September 2020Published
28 August 2020Available
18 August 2020Accepted
Volume: 3
Number: 9
Page Range: pp. 5627-5632
DOI: 10.1021/acsabm.0c00638
Status: Peer Reviewed
Publication Status: Published
Reuse Statement (publisher, data, author rights): ** From Crossref via Jisc Publications Router ** History: epub 28-08-2020; issued 28-08-2020.
Access rights to Published version: Open Access (Creative Commons)
Copyright Holders: Copyright © 2020 American Chemical Society
Date of first compliant deposit: 17 September 2020
Date of first compliant Open Access: 18 September 2020
RIOXX Funder/Project Grant:
Project/Grant IDRIOXX Funder NameFunder ID
StG 638661 H2020 European Research Councilhttp://dx.doi.org/10.13039/100010663
PoC 789182H2020 European Research Councilhttp://dx.doi.org/10.13039/100010663
Industry Fellowship 191037Royal Societyhttp://dx.doi.org/10.13039/501100000288
MC_PC_17203[MRC] Medical Research Councilhttp://dx.doi.org/10.13039/501100000265
EP/R511808/1[EPSRC] Engineering and Physical Sciences Research Councilhttp://dx.doi.org/10.13039/501100000266
EP/ L015307/1RT[EPSRC] Engineering and Physical Sciences Research Councilhttp://dx.doi.org/10.13039/501100000266
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