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The type VII secretion system protects Staphylococcus aureus against antimicrobial host fatty acids
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Kengmo Tchoupa, Arnaud, Watkins, Kate E., Jones, Rebekah A., Kuroki, Agnès, Alam, Mohammad Tauqeer, Perrier, Sébastien, Chen, Yin and Unnikrishnan, Meera (2020) The type VII secretion system protects Staphylococcus aureus against antimicrobial host fatty acids. Scientific Reports, 10 (1). 14838. doi:10.1038/s41598-020-71653-z
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WRAP-The-type-VII-secretion-system-host-fatty-Unnikrishnan-2020.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (2238Kb) | Preview |
Official URL: https://doi.org/10.1038/s41598-020-71653-z
Abstract
The Staphylococcus aureus type VII secretion system (T7SS) exports several proteins that are pivotal for bacterial virulence. The mechanisms underlying T7SS-mediated staphylococcal survival during infection nevertheless remain unclear. Here we report that S. aureus lacking T7SS components are more susceptible to host-derived antimicrobial fatty acids. Unsaturated fatty acids such as linoleic acid (LA) elicited an increased inhibition of S. aureus mutants lacking T7SS effectors EsxC, EsxA and EsxB, or the membrane-bound ATPase EssC, compared to the wild-type (WT). T7SS mutants generated in different S. aureus strain backgrounds also displayed an increased sensitivity to LA. Analysis of bacterial membrane lipid profiles revealed that the esxC mutant was less able to incorporate LA into its membrane phospholipids. Although the ability to bind labelled LA did not differ between the WT and mutant strains, LA induced more cell membrane damage in the T7SS mutants compared to the WT. Furthermore, proteomic analyses of WT and mutant cell fractions revealed that, in addition to compromising membranes, T7SS defects induce oxidative stress and hamper their response to LA challenge. Thus, our findings indicate that T7SS contribute to maintaining S. aureus membrane integrity and homeostasis when bacteria encounter antimicrobial fatty acids.
| Item Type: | Journal Article | |||||||||||||||
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| Subjects: | Q Science > QR Microbiology | |||||||||||||||
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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| SWORD Depositor: | Library Publications Router | |||||||||||||||
| Library of Congress Subject Headings (LCSH): | Biological control systems, Staphylococcus aureus, Unsaturated fatty acids | |||||||||||||||
| Journal or Publication Title: | Scientific Reports | |||||||||||||||
| Publisher: | Nature Publishing Group UK | |||||||||||||||
| ISSN: | 2045-2322 | |||||||||||||||
| Official Date: | 9 September 2020 | |||||||||||||||
| Dates: |
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| Volume: | 10 | |||||||||||||||
| Number: | 1 | |||||||||||||||
| Article Number: | 14838 | |||||||||||||||
| DOI: | 10.1038/s41598-020-71653-z | |||||||||||||||
| Status: | Peer Reviewed | |||||||||||||||
| Publication Status: | Published | |||||||||||||||
| Access rights to Published version: | Open Access | |||||||||||||||
| RIOXX Funder/Project Grant: |
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