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Data for Low DMSO cryopreservation of stem cells enabled by macromolecular cryoprotectants
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Murray, Kathryn, Tomás, Ruben M. F. and Gibson, Matthew I. (2020) Data for Low DMSO cryopreservation of stem cells enabled by macromolecular cryoprotectants. [Dataset]
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WRAP.zip - Published Version Available under License Creative Commons Attribution 4.0. Download (68Kb) |
Abstract
Mesenchymal stromal (stem) cells have potential in regenerative medicine and modulating the immune system. To deliver any cell-based therapy to the patient, it must be cryopreserved, most commonly in DMSO, which impacts cell function and causes clinical side effects. Here we report the use of a synthetically scalable polyampholyte to rescue the cryopreservation of mesenchymal stromal cells in low [DMSO] cryopreservation. Flow cytometry showed retention of key markers of multipotency comparable to 10% (v/v) DMSO, and the cells could be differentiated, showing this polymer material can be used to improve, or replace, current cryopreservation strategies.
Item Type: | Dataset | |||||||||||||||||||||
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Subjects: | Q Science > QD Chemistry Q Science > QH Natural history R Medicine > R Medicine (General) |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | |||||||||||||||||||||
Type of Data: | Experimental data | |||||||||||||||||||||
Library of Congress Subject Headings (LCSH): | Cryopreservation of organs, tissues, etc. , Stem cells -- Cryopreservation, Biomedical materials, Polymers, Polyampholytes, Flow cytometry | |||||||||||||||||||||
Publisher: | University of Warwick, Department of Chemistry | |||||||||||||||||||||
Official Date: | 21 September 2020 | |||||||||||||||||||||
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Status: | Not Peer Reviewed | |||||||||||||||||||||
Publication Status: | Published | |||||||||||||||||||||
Media of Output (format): | .xlsx, .rtf | |||||||||||||||||||||
Access rights to Published version: | Open Access (Creative Commons) | |||||||||||||||||||||
Copyright Holders: | University of Warwick | |||||||||||||||||||||
Description: | Data record consists of a zip archive containing flow cytometry and cell recovery details data, organised according to flow data and freezing data, and an accompanying index file. |
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Date of first compliant deposit: | 21 September 2020 | |||||||||||||||||||||
Date of first compliant Open Access: | 21 September 2020 | |||||||||||||||||||||
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