Murray, Kathryn, Tomás, Ruben M. F. and Gibson, Matthew I. (2020) Data for Low DMSO cryopreservation of stem cells enabled by macromolecular cryoprotectants. [Dataset]

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Abstract

Mesenchymal stromal (stem) cells have potential in regenerative medicine and modulating the immune system. To deliver any cell-based therapy to the patient, it must be cryopreserved, most commonly in DMSO, which impacts cell function and causes clinical side effects. Here we report the use of a synthetically scalable polyampholyte to rescue the cryopreservation of mesenchymal stromal cells in low [DMSO] cryopreservation. Flow cytometry showed retention of key markers of multipotency comparable to 10% (v/v) DMSO, and the cells could be differentiated, showing this polymer material can be used to improve, or replace, current cryopreservation strategies.

Item Type:	Dataset
Divisions:	Faculty of Science > Chemistry
Type of Data:	Experimental data
Publisher:	Department of Chemistry
Official Date:	21 September 2020
Dates:	Date Event 21 September 2020 Created
Date of first compliant deposit:	21 September 2020
Status:	Not Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Open Access
Copyright Holders:	University of Warwick
Description:	Data record consists of a zip archive containing flow cytometry and cell recovery details data, organised according to flow data and freezing data, and an accompanying index file. Flow and freezing data are in excel format. Original cell image files are too large to include here.
RIOXX Funder/Project Grant:	Project/Grant ID RIOXX Funder Name Funder ID StG 638661 H2020 European Research Council http://dx.doi.org/10.13039/100010663 PoC 789182 H2020 European Research Council http://dx.doi.org/10.13039/100010663 Industry Fellowship 191037 Royal Society http://dx.doi.org/10.13039/501100000288 MC_PC_17203 [MRC] Medical Research Council http://dx.doi.org/10.13039/501100000265 EP/R511808/1 [EPSRC] Engineering and Physical Sciences Research Council http://dx.doi.org/10.13039/501100000266 EP/ L015307/1RT [EPSRC] Engineering and Physical Sciences Research Council http://dx.doi.org/10.13039/501100000266
Related URLs:	Related item in WRAP Other
Contributors:	Contribution Name Contributor ID Depositor Gibson, Matthew I. 32015

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