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Data for Low DMSO cryopreservation of stem cells enabled by macromolecular cryoprotectants

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Murray, Kathryn, Tomás, Ruben M. F. and Gibson, Matthew I. (2020) Data for Low DMSO cryopreservation of stem cells enabled by macromolecular cryoprotectants. [Dataset]

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Abstract

Mesenchymal stromal (stem) cells have potential in regenerative medicine and modulating the immune system. To deliver any cell-based therapy to the patient, it must be cryopreserved, most commonly in DMSO, which impacts cell function and causes clinical side effects. Here we report the use of a synthetically scalable polyampholyte to rescue the cryopreservation of mesenchymal stromal cells in low [DMSO] cryopreservation. Flow cytometry showed retention of key markers of multipotency comparable to 10% (v/v) DMSO, and the cells could be differentiated, showing this polymer material can be used to improve, or replace, current cryopreservation strategies.

Item Type: Dataset
Subjects: Q Science > QD Chemistry
Q Science > QH Natural history
R Medicine > R Medicine (General)
Divisions: Faculty of Science > Chemistry
Type of Data: Experimental data
Library of Congress Subject Headings (LCSH): Cryopreservation of organs, tissues, etc. , Stem cells -- Cryopreservation, Biomedical materials, Polymers, Polyampholytes, Flow cytometry
Publisher: Department of Chemistry
Official Date: 21 September 2020
Dates:
DateEvent
21 September 2020Created
Status: Not Peer Reviewed
Publication Status: Published
Media of Output: .xlsx
Access rights to Published version: Open Access
Copyright Holders: University of Warwick
Description:

Data record consists of a zip archive containing flow cytometry and cell recovery details data, organised according to flow data and freezing data, and an accompanying index file.
Flow and freezing data are in excel format. Original cell image files are too large to include here.

RIOXX Funder/Project Grant:
Project/Grant IDRIOXX Funder NameFunder ID
StG 638661 H2020 European Research Councilhttp://dx.doi.org/10.13039/100010663
PoC 789182H2020 European Research Councilhttp://dx.doi.org/10.13039/100010663
Industry Fellowship 191037Royal Societyhttp://dx.doi.org/10.13039/501100000288
MC_PC_17203[MRC] Medical Research Councilhttp://dx.doi.org/10.13039/501100000265
EP/R511808/1[EPSRC] Engineering and Physical Sciences Research Councilhttp://dx.doi.org/10.13039/501100000266
EP/ L015307/1RT[EPSRC] Engineering and Physical Sciences Research Councilhttp://dx.doi.org/10.13039/501100000266
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Contributors:
ContributionNameContributor ID
DepositorGibson, Matthew I.32015

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