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Data for Low DMSO cryopreservation of stem cells enabled by macromolecular cryoprotectants
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Murray, Kathryn, Tomás, Ruben M. F. and Gibson, Matthew I. (2020) Data for Low DMSO cryopreservation of stem cells enabled by macromolecular cryoprotectants. [Dataset]
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Abstract
Mesenchymal stromal (stem) cells have potential in regenerative medicine and modulating the immune system. To deliver any cell-based therapy to the patient, it must be cryopreserved, most commonly in DMSO, which impacts cell function and causes clinical side effects. Here we report the use of a synthetically scalable polyampholyte to rescue the cryopreservation of mesenchymal stromal cells in low [DMSO] cryopreservation. Flow cytometry showed retention of key markers of multipotency comparable to 10% (v/v) DMSO, and the cells could be differentiated, showing this polymer material can be used to improve, or replace, current cryopreservation strategies.
| Item Type: | Dataset | |||||||||||||||||||||
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| Subjects: | Q Science > QD Chemistry Q Science > QH Natural history R Medicine > R Medicine (General) |
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| Divisions: | Faculty of Science > Chemistry | |||||||||||||||||||||
| Type of Data: | Experimental data | |||||||||||||||||||||
| Library of Congress Subject Headings (LCSH): | Cryopreservation of organs, tissues, etc. , Stem cells -- Cryopreservation, Biomedical materials, Polymers, Polyampholytes, Flow cytometry | |||||||||||||||||||||
| Publisher: | Department of Chemistry | |||||||||||||||||||||
| Official Date: | 21 September 2020 | |||||||||||||||||||||
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| Status: | Not Peer Reviewed | |||||||||||||||||||||
| Publication Status: | Published | |||||||||||||||||||||
| Media of Output: | .xlsx | |||||||||||||||||||||
| Access rights to Published version: | Open Access | |||||||||||||||||||||
| Copyright Holders: | University of Warwick | |||||||||||||||||||||
| Description: | Data record consists of a zip archive containing flow cytometry and cell recovery details data, organised according to flow data and freezing data, and an accompanying index file. |
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