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Molecular and functional characterization of somatostatin-type signalling in a deuterostome invertebrate
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Zhang, Ya, Yañez Guerra, Luis Alfonso, Egertová, Michaela, Zampronio, Cleidiane G., Jones, Alexandra M. and Elphick, Maurice R. (2020) Molecular and functional characterization of somatostatin-type signalling in a deuterostome invertebrate. Open Biology, 10 (9). 200172. doi:10.1098/rsob.200172 ISSN 2046-2441.
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WRAP-Molecular-functional-characterization-somatostatin-type-deuterostome-Jones-2020.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (3250Kb) | Preview |
Official URL: https://doi.org/10.1098/rsob.200172
Abstract
Somatostatin (SS) and allatostatin-C (ASTC) are structurally and evolutionarily related neuropeptides that act as inhibitory regulators of physiological processes in mammals and insects, respectively. Here, we report the first molecular and functional characterization of SS/ASTC-type signalling in a deuterostome invertebrate-the starfish (phylum Echinodermata). Two SS/ASTC-type precursors were identified in (ArSSP1 and ArSSP2) and the structures of neuropeptides derived from these proteins (ArSS1 and ArSS2) were analysed using mass spectrometry. Pharmacological characterization of three cloned SS/ASTC-type receptors (ArSSR1-3) revealed that ArSS2, but not ArSS1, acts as a ligand for all three receptors. Analysis of ArSS2 expression in using mRNA hybridization and immunohistochemistry revealed stained cells/fibres in the central nervous system, the digestive system (e.g. cardiac stomach) and the body wall and its appendages (e.g. tube feet). Furthermore, pharmacological tests revealed that ArSS2 causes dose-dependent relaxation of tube foot and cardiac stomach preparations, while injection of ArSS2 causes partial eversion of the cardiac stomach. Our findings provide new insights into the molecular evolution of SS/ASTC-type signalling in the animal kingdom and reveal an ancient role of SS-type neuropeptides as inhibitory regulators of muscle contractility.
Item Type: | Journal Article | |||||||||||||||||||||
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Subjects: | Q Science > QL Zoology Q Science > QP Physiology |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | |||||||||||||||||||||
SWORD Depositor: | Library Publications Router | |||||||||||||||||||||
Library of Congress Subject Headings (LCSH): | Neuropeptides -- Receptors, Somatostatin -- Receptors, Neuropeptides -- Evolution, Starfishes, Muscle contraction | |||||||||||||||||||||
Journal or Publication Title: | Open Biology | |||||||||||||||||||||
Publisher: | The Royal Society Publishing | |||||||||||||||||||||
ISSN: | 2046-2441 | |||||||||||||||||||||
Official Date: | 9 September 2020 | |||||||||||||||||||||
Dates: |
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Volume: | 10 | |||||||||||||||||||||
Number: | 9 | |||||||||||||||||||||
Article Number: | 200172 | |||||||||||||||||||||
DOI: | 10.1098/rsob.200172 | |||||||||||||||||||||
Status: | Peer Reviewed | |||||||||||||||||||||
Publication Status: | Published | |||||||||||||||||||||
Reuse Statement (publisher, data, author rights): | ** From PubMed via Jisc Publications Router | |||||||||||||||||||||
Access rights to Published version: | Open Access (Creative Commons) | |||||||||||||||||||||
Date of first compliant deposit: | 15 October 2020 | |||||||||||||||||||||
Date of first compliant Open Access: | 15 October 2020 | |||||||||||||||||||||
RIOXX Funder/Project Grant: |
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