The Library
Data for Ex vivo electrochemical pH mapping of the gastrointestinal tract in the absence and presence of pharmacological agents
Tools
Rajan, Teena S., Read, Tania L., Abdalla, Aya, Patel, Bhavik A. and Macpherson, Julie V. (2020) Data for Ex vivo electrochemical pH mapping of the gastrointestinal tract in the absence and presence of pharmacological agents. [Dataset]
Archive (ZIP) (Zip archive)
WRAP (1).zip - Published Version Available under License Creative Commons Attribution 4.0. Download (80Mb) |
|
Plain Text (Readme file)
Read me.docx - Published Version Available under License Creative Commons Attribution 4.0. Download (13Kb) |
Abstract
Ex vivo pH profiling of the upper gastrointestinal (GI) tract (of a mouse), using an electrochemical pH probe, in both the absence and presence of pharmacological agents aimed at altering acid/bicarbonate production, is reported. Three pH electrodes were first assessed for suitability using a GI tract biological mimic buffer solution containing 0.5% mucin. These include a traditional glass pH probe, an iridium oxide (IrOx)-coated electrode (both operated potentiometrically), and a quinone (Q) surface-integrated boron-doped diamond (BDD-Q) electrode (voltammetric). In mucin, the time scale for both IrOx and glass to provide a representative pH reading was in the ∼100’s of s, most likely due to mucin adsorption, in contrast to 6 s with the BDD-Q electrode. Both the glass and IrOx pH electrodes were also compromised on robustness due to fragility and delamination (IrOx) issues; contact with the GI tissue was an experimental requirement. BDD-Q was deemed the most appropriate. Ten measurements were made along the GI tract, esophagus (1), stomach (5), and duodenum (4). Under buffer only conditions, the BDD-Q probe tracked the pH from neutral in the esophagus to acidic in the stomach and rising to more alkaline in the duodenum. In the presence of omeprazole, a proton pump inhibitor, the body regions of the stomach exhibited elevated pH levels. Under melatonin treatment (a bicarbonate agonist and acid inhibitor), both the body of the stomach and the duodenum showed elevated pH levels. This study demonstrates the versatility of the BDD-Q pH electrode for real-time ex vivo biological tissue measurements.
Item Type: | Dataset | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Subjects: | Q Science > QD Chemistry Q Science > QH Natural history Q Science > QP Physiology |
|||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | |||||||||
Type of Data: | Experimental data | |||||||||
Library of Congress Subject Headings (LCSH): | Scanning electrochemical microscopy, Hydrogen-ion concentration -- Measurement, Gastrointestinal system | |||||||||
Publisher: | University of Warwick, Department of Chemistry | |||||||||
Official Date: | 13 October 2020 | |||||||||
Dates: |
|
|||||||||
Status: | Not Peer Reviewed | |||||||||
Publication Status: | Published | |||||||||
Media of Output (format): | .txt, .nox, .png, .pdf, .opju, .xlsx, .pzfx, .py, .csv, .bin, .tif | |||||||||
Access rights to Published version: | Open Access (Creative Commons) | |||||||||
Copyright Holders: | University of Warwick | |||||||||
Description: | Data record consists of a zip archive, containing raw data organised into subfolders according to data type, and an accompanying readme file. |
|||||||||
Date of first compliant deposit: | 14 October 2020 | |||||||||
Date of first compliant Open Access: | 14 October 2020 | |||||||||
RIOXX Funder/Project Grant: |
|
|||||||||
Related URLs: | ||||||||||
Contributors: |
|
Request changes or add full text files to a record
Repository staff actions (login required)
View Item |
Downloads
Downloads per month over past year