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The SARS-COV-2 spike protein binds sialic acids and enables rapid detection in a lateral flow point of care diagnostic device
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Baker, Alexander, Richards, Sarah-Jane, Guy, Collette S., Congdon, Thomas R., Hasan, Muhammad, Zwetsloot, Alexander J., Gallo, Angelo, Lewandowski, Józef R., Stansfeld, Phillip J., Straube, Anne, Walker, Marc, Chessa, Simona, Pergolizzi, Giulia, Dedola, Simone, Field, Robert A. and Gibson, Matthew I. (2020) The SARS-COV-2 spike protein binds sialic acids and enables rapid detection in a lateral flow point of care diagnostic device. ACS Central Science, 6 (11). pp. 2046-2052. doi:10.1021/acscentsci.0c00855 ISSN 2374-7951.
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Official URL: https://doi.org/10.1021/acscentsci.0c00855
Abstract
There is an urgent need to understand the behavior of the novel coronavirus (SARS-COV-2), which is the causative agent of COVID-19, and to develop point-of-care diagnostics. Here, a glyconanoparticle platform is used to discover that N-acetyl neuraminic acid has affinity toward the SARS-COV-2 spike glycoprotein, demonstrating its glycan-binding function. Optimization of the particle size and coating enabled detection of the spike glycoprotein in lateral flow and showed selectivity over the SARS-COV-1 spike protein. Using a virus-like particle and a pseudotyped lentivirus model, paper-based lateral flow detection was demonstrated in under 30 min, showing the potential of this system as a low-cost detection platform.
| Item Type: | Journal Article | ||||||||||||||||||||||||||||||||||||||||||
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| Subjects: | R Medicine > RA Public aspects of medicine > RA0421 Public health. Hygiene. Preventive Medicine R Medicine > RC Internal medicine |
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| Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) Faculty of Science, Engineering and Medicine > Science > Physics Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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| Library of Congress Subject Headings (LCSH): | COVID-19 (Disease) -- Diagnosis, Point-of-care testing, Glycoproteins, Sialic acids | ||||||||||||||||||||||||||||||||||||||||||
| Journal or Publication Title: | ACS Central Science | ||||||||||||||||||||||||||||||||||||||||||
| Publisher: | ACS | ||||||||||||||||||||||||||||||||||||||||||
| ISSN: | 2374-7951 | ||||||||||||||||||||||||||||||||||||||||||
| Official Date: | 25 November 2020 | ||||||||||||||||||||||||||||||||||||||||||
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| Volume: | 6 | ||||||||||||||||||||||||||||||||||||||||||
| Number: | 11 | ||||||||||||||||||||||||||||||||||||||||||
| Page Range: | pp. 2046-2052 | ||||||||||||||||||||||||||||||||||||||||||
| DOI: | 10.1021/acscentsci.0c00855 | ||||||||||||||||||||||||||||||||||||||||||
| Status: | Peer Reviewed | ||||||||||||||||||||||||||||||||||||||||||
| Publication Status: | Published | ||||||||||||||||||||||||||||||||||||||||||
| Access rights to Published version: | Open Access (Creative Commons) | ||||||||||||||||||||||||||||||||||||||||||
| Copyright Holders: | Copyright © 2020 American Chemical Society | ||||||||||||||||||||||||||||||||||||||||||
| Date of first compliant deposit: | 30 September 2020 | ||||||||||||||||||||||||||||||||||||||||||
| Date of first compliant Open Access: | 5 October 2020 | ||||||||||||||||||||||||||||||||||||||||||
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