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Examining local cell-to-cell signalling in the kidney using ATP biosensing
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Price, Gareth W., Potter, Joe A., Williams, Bethany M., Cliff, Chelsy L., Wall, Mark J., Hills, Claire E. and Squires, Paul E. (2020) Examining local cell-to-cell signalling in the kidney using ATP biosensing. In: Turksen, K., (ed.) Stem Cell Renewal and Cell-Cell Communication. Methods in Molecular Biology, 2346 . New York: Humana Press, pp. 135-149. ISBN 9781071615690
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Official URL: http://dx.doi.org/10.1007/7651_2020_297
Abstract
Cell-to-cell communication is an essential process for the efficient function of cells and tissues. Central to this is the purinergic transmission of purines, with ligands such as adenosine triphosphate (ATP). Altered cell-to-cell communication, and in particular changes in the paracrine release of extracellular ATP, plays crucial roles in pathophysiological conditions, such as diabetes. ATP biosensing provides a reliable, real-time measurement of local extracellular ATP concentrations. This allows the detection of altered ATP release, which underlies the progression of inflammation and fibrosis and is a potential therapeutic target. Here we describe in a step-by-step basis how to utilize sensitive microelectrode biosensors to detect low, real-time concentrations of ATP, in vitro.
Item Type: | Book Item | ||||||
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||
Series Name: | Methods in Molecular Biology | ||||||
Publisher: | Humana Press | ||||||
Place of Publication: | New York | ||||||
ISBN: | 9781071615690 | ||||||
ISSN: | 1064-3745 | ||||||
Book Title: | Stem Cell Renewal and Cell-Cell Communication | ||||||
Editor: | Turksen, K. | ||||||
Official Date: | 14 July 2020 | ||||||
Dates: |
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Volume: | 2346 | ||||||
Page Range: | pp. 135-149 | ||||||
DOI: | 10.1007/7651_2020_297 | ||||||
Status: | Peer Reviewed | ||||||
Publication Status: | Published | ||||||
Access rights to Published version: | Restricted or Subscription Access |
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