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Detecting CO2-sensitive hemichannels in neurons in acute brain slices

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Hill, Emily, Dale, Nicholas and Wall, Mark J. (2020) Detecting CO2-sensitive hemichannels in neurons in acute brain slices. STAR Protocols, 1 (3). 100139. doi:10.1016/j.xpro.2020.100139 ISSN 2666-1667.

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Official URL: https://doi.org/10.1016/j.xpro.2020.100139

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Abstract

This protocol provides two independent methods to functionally detect the neuronal expression of CO2-sensitive hemichannels. These hemichannels (consisting of connexins 26 or 30) are directly gated by CO2, independent of pH changes and until recently were thought to be only expressed by glia. This protocol outlines a method to change the concentration of CO2 without changing pH, using isohydric solutions and then utilizing this to detect opening and closing of functional hemichannels using whole-cell patch clamp recording and dye loading.

Item Type: Journal Article
Subjects: Q Science > QP Physiology
Divisions: Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- )
Library of Congress Subject Headings (LCSH): Neurons, Astrocytes, Nervous system, Brain -- Physiology, Connexins
Journal or Publication Title: STAR Protocols
Publisher: Cell Press
ISSN: 2666-1667
Official Date: 18 December 2020
Dates:
DateEvent
18 December 2020Published
23 October 2020Available
30 September 2020Accepted
Volume: 1
Number: 3
Article Number: 100139
DOI: 10.1016/j.xpro.2020.100139
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Open Access (Creative Commons)
Date of first compliant deposit: 12 October 2020
Date of first compliant Open Access: 14 October 2020
RIOXX Funder/Project Grant:
Project/Grant IDRIOXX Funder NameFunder ID
UNSPECIFIED[BBSRC] Biotechnology and Biological Sciences Research Councilhttp://dx.doi.org/10.13039/501100000268
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