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Ex vivo electrochemical pH mapping of the gastrointestinal tract in the absence and presence of pharmacological agents
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Rajan, Teena S., Read, Tania L., Abdalla, Aya, Patel, Bhavik A. and Macpherson, Julie V. (2020) Ex vivo electrochemical pH mapping of the gastrointestinal tract in the absence and presence of pharmacological agents. ACS Sensors, 5 (9). pp. 2858-2865. doi:10.1021/acssensors.0c01020 ISSN 2379-3694.
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WRAP-Ex-vivo-electrochemical-pH-mapping-gastrointestinal-absence-presence-pharmacological-.pdf - Accepted Version - Requires a PDF viewer. Download (1299Kb) | Preview |
Official URL: https://doi.org/10.1021/acssensors.0c01020
Abstract
Ex vivo pH profiling of the upper gastrointestinal (GI) tract (of a mouse), using an electrochemical pH probe, in both the absence and presence of pharmacological agents aimed at altering acid/bicarbonate production, is reported. Three pH electrodes were first assessed for suitability using a GI tract biological mimic buffer solution containing 0.5% mucin. These include a traditional glass pH probe, an iridium oxide (IrOx)-coated electrode (both operated potentiometrically), and a quinone (Q) surface-integrated boron-doped diamond (BDD-Q) electrode (voltammetric). In mucin, the time scale for both IrOx and glass to provide a representative pH reading was in the ∼100’s of s, most likely due to mucin adsorption, in contrast to 6 s with the BDD-Q electrode. Both the glass and IrOx pH electrodes were also compromised on robustness due to fragility and delamination (IrOx) issues; contact with the GI tissue was an experimental requirement. BDD-Q was deemed the most appropriate. Ten measurements were made along the GI tract, esophagus (1), stomach (5), and duodenum (4). Under buffer only conditions, the BDD-Q probe tracked the pH from neutral in the esophagus to acidic in the stomach and rising to more alkaline in the duodenum. In the presence of omeprazole, a proton pump inhibitor, the body regions of the stomach exhibited elevated pH levels. Under melatonin treatment (a bicarbonate agonist and acid inhibitor), both the body of the stomach and the duodenum showed elevated pH levels. This study demonstrates the versatility of the BDD-Q pH electrode for real-time ex vivo biological tissue measurements.
Item Type: | Journal Article | |||||||||
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Subjects: | Q Science > QD Chemistry Q Science > QH Natural history Q Science > QP Physiology |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | |||||||||
Library of Congress Subject Headings (LCSH): | Scanning electrochemical microscopy, Hydrogen-ion concentration -- Measurement, Gastrointestinal system | |||||||||
Journal or Publication Title: | ACS Sensors | |||||||||
Publisher: | American Chemical Society | |||||||||
ISSN: | 2379-3694 | |||||||||
Official Date: | 25 September 2020 | |||||||||
Dates: |
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Volume: | 5 | |||||||||
Number: | 9 | |||||||||
Page Range: | pp. 2858-2865 | |||||||||
DOI: | 10.1021/acssensors.0c01020 | |||||||||
Status: | Peer Reviewed | |||||||||
Publication Status: | Published | |||||||||
Reuse Statement (publisher, data, author rights): | “This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Sensors, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see [insert ACS Articles on Request author-directed link to Published Work, see http://pubs.acs.org/page/policy/articlesonrequest/index.html].” | |||||||||
Access rights to Published version: | Restricted or Subscription Access | |||||||||
Copyright Holders: | Copyright © 2020 American Chemical Society | |||||||||
Date of first compliant deposit: | 20 October 2020 | |||||||||
Date of first compliant Open Access: | 7 July 2021 | |||||||||
RIOXX Funder/Project Grant: |
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