The Library
Real-time measurement of adenosine and ATP release in the central nervous system
Tools
Tools
Tools
Dale, Nicholas (2021) Real-time measurement of adenosine and ATP release in the central nervous system. Purinergic Signalling, 17 (1). pp. 109-115. doi:10.1007/s11302-020-09733-y
|
PDF
WRAP-Real-time-measurement-adenosine-ATP-central-nervous-Dale-2020.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (1275Kb) | Preview |
Official URL: https://doi.org/10.1007/s11302-020-09733-y
Abstract
This brief review recounts how, stimulated by the work of Geoff Burnstock, I developed biosensors that allowed direct real-time measurement of ATP and adenosine during neural function. The initial impetus to create an adenosine biosensor came from trying to understand how ATP and adenosine-modulated motor pattern generation in the frog embryo spinal cord. Early biosensor measurements demonstrated slow accumulation of adenosine during motor activity. Subsequent application of these biosensors characterized real-time release of adenosine in in vitro models of brain ischaemia, and this line of work has recently led to clinical measurements of whole blood purine levels in patients undergoing carotid artery surgery or stroke. In parallel, the wish to understand the role of ATP signalling in the chemosensory regulation of breathing stimulated the development of ATP biosensors. This revealed that release of ATP from the chemosensory areas of the medulla oblongata preceded adaptive changes in breathing, triggered adaptive changes in breathing via activation of P2 receptors, and ultimately led to the discovery of connexin26 as a channel that mediates CO2-gated release of ATP from cells.
| Item Type: | Journal Article | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Subjects: | Q Science > QP Physiology R Medicine > R Medicine (General) |
||||||||||||
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||||||
| SWORD Depositor: | Library Publications Router | ||||||||||||
| Library of Congress Subject Headings (LCSH): | Biosensors -- Design, Adenosine triphosphate -- Measurement | ||||||||||||
| Journal or Publication Title: | Purinergic Signalling | ||||||||||||
| Publisher: | Springer Netherlands | ||||||||||||
| ISSN: | 1573-9538 | ||||||||||||
| Official Date: | March 2021 | ||||||||||||
| Dates: |
|
||||||||||||
| Volume: | 17 | ||||||||||||
| Number: | 1 | ||||||||||||
| Page Range: | pp. 109-115 | ||||||||||||
| DOI: | 10.1007/s11302-020-09733-y | ||||||||||||
| Status: | Peer Reviewed | ||||||||||||
| Publication Status: | Published | ||||||||||||
| Access rights to Published version: | Open Access | ||||||||||||
| RIOXX Funder/Project Grant: |
|
Request changes or add full text files to a record
Repository staff actions (login required)
 |
View Item |
Downloads
Downloads per month over past year
