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A method for identifying G protein-coupled receptor dimers and their interfaces

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Chen, Jing, Cai, Xin, Yan, Maocai, Wang, Zhengwen, Lv, Zhitong and Wang, Chunmei (2020) A method for identifying G protein-coupled receptor dimers and their interfaces. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research . 118887. doi:10.1016/j.bbamcr.2020.118887 (In Press)

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Official URL: http://dx.doi.org/10.1016/j.bbamcr.2020.118887

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Abstract

The G protein-coupled receptor (GPCR) dimer interface plays an important role in the formation and stabilization of the dimer. Therefore, identifying the potential receptor-receptor interface is an important part of studying GPCRs. Various strategies have been employed to study the GPCR dimer interface and explore its functional significance, but experimental methods lack robustness and calculations are laborious. Herein, we report a combined optimized experimental and calculation approach for identifying and structurally characterizing GPCR dimer interfaces, and constructing atomic resolution models. Using a transmembrane domain (TM) peptide containing a human immunodeficiency virus trans-acting transcriptional activator (HIV-TAT) protein transduction motif, matrix-assisted laser desorption tandem time-of-flight mass spectrometry (MALDITOF-MS), and bioluminescence resonance energy transfer (BRET), we successfully identified Apelin receptor (APJ)/Nociceptin receptor 1 (ORL1) and APJ/Vasopressin receptor 2 (V2R) heterodimer interfaces, and two key sites mediating dimerization. This method can identify dimer interfaces of GPCR homodimers and heterodimers.

Item Type: Journal Article
Subjects: Q Science > QD Chemistry
Q Science > QP Physiology
Divisions: Faculty of Medicine > Warwick Medical School > Biomedical Sciences
Faculty of Medicine > Warwick Medical School > Health Sciences
Faculty of Medicine > Warwick Medical School
Library of Congress Subject Headings (LCSH): G proteins , G proteins -- Receptors, Dimers, Membrane proteins -- Metabolism, Mass spectrometry, Bioluminescence assay
Journal or Publication Title: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research
Publisher: Elsevier
ISSN: 0167-4889
Official Date: 16 October 2020
Dates:
DateEvent
16 October 2020Available
8 October 2020Accepted
Date of first compliant deposit: 23 October 2020
Article Number: 118887
DOI: 10.1016/j.bbamcr.2020.118887
Status: Peer Reviewed
Publication Status: In Press
Access rights to Published version: Open Access
RIOXX Funder/Project Grant:
Project/Grant IDRIOXX Funder NameFunder ID
31271243[NSFC] National Natural Science Foundation of Chinahttp://dx.doi.org/10.13039/501100001809
ZR2018MC005Natural Science Foundation of Shandong Provincehttp://dx.doi.org/10.13039/501100007129
ZR2018PC007Natural Science Foundation of Shandong Provincehttp://dx.doi.org/10.13039/501100007129
JYP2018KJ18Jining Medical Universityhttp://dx.doi.org/10.13039/501100008853

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