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A method for identifying G protein-coupled receptor dimers and their interfaces
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Chen, Jing, Cai, Xin, Yan, Maocai, Wang, Zhengwen, Lv, Zhitong and Wang, Chunmei (2020) A method for identifying G protein-coupled receptor dimers and their interfaces. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research . 118887. doi:10.1016/j.bbamcr.2020.118887 (In Press)
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WRAP-method-identifying-G-protein-coupled-receptor-dimers-interfaces-Chen-2020.pdf - Accepted Version Embargoed item. Restricted access to Repository staff only until 16 October 2021. Contact author directly, specifying your specific needs. - Requires a PDF viewer. Available under License Creative Commons Attribution Non-commercial No Derivatives 4.0. Download (1901Kb) |
Official URL: http://dx.doi.org/10.1016/j.bbamcr.2020.118887
Abstract
The G protein-coupled receptor (GPCR) dimer interface plays an important role in the formation and stabilization of the dimer. Therefore, identifying the potential receptor-receptor interface is an important part of studying GPCRs. Various strategies have been employed to study the GPCR dimer interface and explore its functional significance, but experimental methods lack robustness and calculations are laborious. Herein, we report a combined optimized experimental and calculation approach for identifying and structurally characterizing GPCR dimer interfaces, and constructing atomic resolution models. Using a transmembrane domain (TM) peptide containing a human immunodeficiency virus trans-acting transcriptional activator (HIV-TAT) protein transduction motif, matrix-assisted laser desorption tandem time-of-flight mass spectrometry (MALDITOF-MS), and bioluminescence resonance energy transfer (BRET), we successfully identified Apelin receptor (APJ)/Nociceptin receptor 1 (ORL1) and APJ/Vasopressin receptor 2 (V2R) heterodimer interfaces, and two key sites mediating dimerization. This method can identify dimer interfaces of GPCR homodimers and heterodimers.
| Item Type: | Journal Article | |||||||||||||||
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| Subjects: | Q Science > QD Chemistry Q Science > QP Physiology |
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| Divisions: | Faculty of Medicine > Warwick Medical School > Biomedical Sciences Faculty of Medicine > Warwick Medical School > Health Sciences Faculty of Medicine > Warwick Medical School |
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| Library of Congress Subject Headings (LCSH): | G proteins , G proteins -- Receptors, Dimers, Membrane proteins -- Metabolism, Mass spectrometry, Bioluminescence assay | |||||||||||||||
| Journal or Publication Title: | Biochimica et Biophysica Acta (BBA) - Molecular Cell Research | |||||||||||||||
| Publisher: | Elsevier | |||||||||||||||
| ISSN: | 0167-4889 | |||||||||||||||
| Official Date: | 16 October 2020 | |||||||||||||||
| Dates: |
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| Date of first compliant deposit: | 23 October 2020 | |||||||||||||||
| Article Number: | 118887 | |||||||||||||||
| DOI: | 10.1016/j.bbamcr.2020.118887 | |||||||||||||||
| Status: | Peer Reviewed | |||||||||||||||
| Publication Status: | In Press | |||||||||||||||
| Access rights to Published version: | Open Access | |||||||||||||||
| RIOXX Funder/Project Grant: |
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