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Exploring selective autophagy in Drosophila : methods to identify Atg8-interacting proteins
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Petridi, Stavroula, Jacomin, Anne-Claire, Bhujabal, Zambarla, Johansen, Terje and Nezis, Ioannis P. (2021) Exploring selective autophagy in Drosophila : methods to identify Atg8-interacting proteins. In: Methods in Cell Biology. Elsevier, pp. 13-29. ISBN 9780128244876
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Official URL: https://doi.org/10.1016/bs.mcb.2020.10.008
Abstract
Autophagy has been described as a catabolic process in which cytoplasmic material is being recycled under various conditions of cellular stress, preventing cell damage and promoting cell survival. Drosophila has been demonstrated to provide an excellent animal model for the study of autophagy. Here we provide a detailed experimental procedure for the identification of Atg8a interactors, exploiting the iLIR database, followed by the in vitro confirmation of interactions and in situ detection of the respective proteins.
Item Type: | Book Item | |||||||||
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | |||||||||
Journal or Publication Title: | Methods in Cell Biology | |||||||||
Publisher: | Elsevier | |||||||||
ISBN: | 9780128244876 | |||||||||
ISSN: | 0091-679X | |||||||||
Book Title: | Methods in Cell Biology | |||||||||
Official Date: | July 2021 | |||||||||
Dates: |
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Volume: | 165 | |||||||||
Page Range: | pp. 13-29 | |||||||||
DOI: | 10.1016/bs.mcb.2020.10.008 | |||||||||
Status: | Peer Reviewed | |||||||||
Publication Status: | Published | |||||||||
Access rights to Published version: | Restricted or Subscription Access | |||||||||
Copyright Holders: | Copyright © 2020 Elsevier Inc. All rights reserved. | |||||||||
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