Three-dimensional structure of Aleutian mink disease parvovirus: Implications for disease pathogenicity
UNSPECIFIED. (1999) Three-dimensional structure of Aleutian mink disease parvovirus: Implications for disease pathogenicity. JOURNAL OF VIROLOGY, 73 (8). pp. 6882-6891. ISSN 0022-538XFull text not available from this repository.
The three-dimensional structure of expressed VP2 capsids of Aleutian mink disease parvovirus strain G (ADV(G-VPZ)) has been determined to 22 Angstrom resolution by cryo-electron microscopy and image reconstruction techniques. A structure-based sequence alignment of the VP2 capsid protein of canine parvovirus (CPV) provided a means to construct an atomic model of the ADV(G-VP2) capsid. The ADV(G-VP2) reconstruction reveals a capsid structure with a mean external radius of 128 Angstrom and several surface features similar to those found in human parvovirus B19 (B19), CPV, feline panleukopenia virus (FPV), and minute virus of mice (MVM). Dimple-like depressions occur at the icosahedral twofold axes, canyon-like regions encircle the fivefold axes, and spike-like protrusions decorate the threefold axes. These spikes are not present in B19, and they are more prominent in ADV compared to the other parvoviruses owing to the presence of loop insertions which create mounds near the threefold axes. Cylindrical channels along the fivefold axes of CPV, FPV, and MVM, which are surrounded by five symmetry-related beta-ribbons, are closed in ADV(G-VP2) and B19. Immunoreactive peptides made from segments of the ADV(G-VP2) capsid protein map to residues in the mound structures. In vitro tissue tropism and in vivo pathogenic properties of ADV map to residues at the threefold axes and to the wall of the dimples.
|Item Type:||Journal Article|
|Subjects:||Q Science > QR Microbiology > QR355 Virology|
|Journal or Publication Title:||JOURNAL OF VIROLOGY|
|Publisher:||AMER SOC MICROBIOLOGY|
|Official Date:||August 1999|
|Number of Pages:||10|
|Page Range:||pp. 6882-6891|
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