The Library
Engineered RNA-interacting CRISPR guide RNAs for genetic sensing and diagnostics
Tools
Tools
Tools
Galizi, Roberto, Duncan, John, Rostain, William, Quinn, Charlotte M., Storch, Marko, Kushwaha, Manish and Jaramillo, Alfonso (2020) Engineered RNA-interacting CRISPR guide RNAs for genetic sensing and diagnostics. The CRISPR Journal, 3 (5). pp. 398-408. doi:10.1089/crispr.2020.0029 ISSN 2573-1602.
Research output not available from this repository.
Request-a-Copy directly from author or use local Library Get it For Me service.
Official URL: https://doi.org/10.1089/crispr.2020.0029
Abstract
CRISPR guide RNAs (gRNAs) can be programmed with relative ease to allow the genetic editing of nearly any DNA or RNA sequence. Here, we propose novel molecular architectures to achieve RNA-dependent modulation of CRISPR activity in response to specific RNA molecules. We designed and tested, in both living Escherichia coli cells and cell-free assays for rapid prototyping, cis-repressed RNA-interacting guide RNA (igRNA) that switch to their active state only upon interaction with small RNA fragments or long RNA transcripts, including pathogen-derived mRNAs of medical relevance such as the human immunodeficiency virus infectivity factor. The proposed CRISPR-igRNAs are fully customizable and easily adaptable to the majority if not all the available CRISPR-Cas variants to modulate a variety of genetic functions in response to specific cellular conditions, providing orthogonal activation and increased specificity. We thereby foresee a large scope of application for therapeutic, diagnostic, and biotech applications in both prokaryotic and eukaryotic systems.
| Item Type: | Journal Article | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||
| SWORD Depositor: | Library Publications Router | ||||||||
| Journal or Publication Title: | The CRISPR Journal | ||||||||
| Publisher: | Mary Ann Liebert Inc | ||||||||
| ISSN: | 2573-1602 | ||||||||
| Official Date: | 20 October 2020 | ||||||||
| Dates: |
|
||||||||
| Volume: | 3 | ||||||||
| Number: | 5 | ||||||||
| Page Range: | pp. 398-408 | ||||||||
| DOI: | 10.1089/crispr.2020.0029 | ||||||||
| Status: | Peer Reviewed | ||||||||
| Publication Status: | Published | ||||||||
| Access rights to Published version: | Open Access (Creative Commons) | ||||||||
| Copyright Holders: | Copyright 2020, Mary Ann Liebert, Inc., publishers | ||||||||
| Description: | Free access but no license on reuse |
Request changes or add full text files to a record
Repository staff actions (login required)
 |
View Item |
