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Flow rate controlled pipetting for microfluidics : second generation flexible hydraulic reservoir (FHRv2)
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Atay, Atakan, Topuz, Alper, Sarıarslana, Busra, Yıldırım, Ender, Charmet, Jérôme, Couling, Kevin and Cetin, Barbaros (2021) Flow rate controlled pipetting for microfluidics : second generation flexible hydraulic reservoir (FHRv2). Microfluidics and Nanofluidics, 25 . 3. doi:10.1007/s10404-020-02402-x ISSN 1613-4982.
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WRAP-Flow-rate-controlled-pipetting-microfluidics-second-flexible-hydraulic-reservoir-Charmet-2020.pdf - Accepted Version - Requires a PDF viewer. Download (9Mb) | Preview |
Official URL: https://doi.org/10.1007/s10404-020-02402-x
Abstract
A critical component of microfluidic technology is the fluid pumping mechanism. Syringe and pressure pumps are typically used in the lab environment; however, their operations generate considerable dead volume that is often larger than the volume of the chip itself, leading to considerable waste of precious sample. As an alternative, pipetting allows for precise liquid dispensing with zero dead volume; however, it has a limited flow control. Recently, we have introduced a low-cost sample loading interface with zero dead-volume named flexible hydraulic reservoir (FHR). In this study, we present a second-generation FHRv2 that combines continuous pumping, zero-dead volume and the versatility of pipetting. The performance of FHRv2 is tested against a syringe pump at flow rates ranging between 20 and 60 μL/min. It demonstrated smoother operation and identical transient time to reach steady flow rate as confirmed by a mathematical model developed for the occasion. Importantly, we also demonstrate that the FHRv2 prevents sedimentation-induced artifacts typically encountered in typical syringe pumps when dispensing particles. Finally, we demonstrate the fabrication of the FHRv2 concept with injection molding using a 3D-printed mold. Overall, our FHRv2 offers a low-cost and versatile solution for zero-volume liquid handling in microfluidic devices.
| Item Type: | Journal Article | ||||||||||||
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| Subjects: | T Technology > TJ Mechanical engineering and machinery T Technology > TS Manufactures |
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| Divisions: | Faculty of Science, Engineering and Medicine > Engineering > WMG (Formerly the Warwick Manufacturing Group) | ||||||||||||
| Library of Congress Subject Headings (LCSH): | Microfluidics, Process control | ||||||||||||
| Journal or Publication Title: | Microfluidics and Nanofluidics | ||||||||||||
| Publisher: | Springer | ||||||||||||
| ISSN: | 1613-4982 | ||||||||||||
| Official Date: | 4 January 2021 | ||||||||||||
| Dates: |
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| Volume: | 25 | ||||||||||||
| Article Number: | 3 | ||||||||||||
| DOI: | 10.1007/s10404-020-02402-x | ||||||||||||
| Status: | Peer Reviewed | ||||||||||||
| Publication Status: | Published | ||||||||||||
| Access rights to Published version: | Restricted or Subscription Access | ||||||||||||
| Date of first compliant deposit: | 12 November 2020 | ||||||||||||
| Date of first compliant Open Access: | 4 January 2022 | ||||||||||||
| RIOXX Funder/Project Grant: |
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