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Campylobacter jejuni 11168H exposed to penicillin forms persister cells and cells with altered redox protein activity
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Morcrette, Helen, Kovacs-Simon, Andrea, Tennant, Richard K., Love, John, Wagley, Sariqa, Yang, Zheng R., Studholme, David J., Soyer, Orkun S., Champion, Olivia L., Butler, Clive S. and Titball, Richard W. (2020) Campylobacter jejuni 11168H exposed to penicillin forms persister cells and cells with altered redox protein activity. Frontiers in Cellular and Infection Microbiology, 10 . 565975. doi:10.3389/fcimb.2020.565975
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WRAP-campylobacter-jejuni-11168H-exposed-penicillin-forms-persister-cells-altered-redox-protein-activity-Soyer-2020.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (2228Kb) | Preview |
Official URL: http://dx.doi.org/10.3389/fcimb.2020.565975
Abstract
The formation of persister cells is one mechanism by which bacteria can survive exposure to environmental stresses. We show that Campylobacter jejuni 11168H forms persister cells at a frequency of 10−3 after exposure to 100 × MIC of penicillin G for 24 h. Staining the cell population with a redox sensitive fluorescent dye revealed that penicillin G treatment resulted in the appearance of a population of cells with increased fluorescence. We present evidence, to show this could be a consequence of increased redox protein activity in, or associated with, the electron transport chain. These data suggest that a population of penicillin G treated C. jejuni cells could undergo a remodeling of the electron transport chain in order to moderate membrane hyperpolarization and intracellular alkalization; thus reducing the antibiotic efficacy and potentially assisting in persister cell formation.
| Item Type: | Journal Article | ||||||
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| Subjects: | Q Science > QH Natural history Q Science > QP Physiology Q Science > QR Microbiology R Medicine > RM Therapeutics. Pharmacology |
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| Divisions: | Faculty of Science > Life Sciences (2010- ) | ||||||
| Library of Congress Subject Headings (LCSH): | Campylobacter jejuni , Proteomics, Drug resistance in microorganisms, Antibiotics, Electron transport | ||||||
| Journal or Publication Title: | Frontiers in Cellular and Infection Microbiology | ||||||
| Publisher: | Frontiers Media | ||||||
| ISSN: | 2235-2988 | ||||||
| Official Date: | 19 October 2020 | ||||||
| Dates: |
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| Date of first compliant deposit: | 16 November 2020 | ||||||
| Volume: | 10 | ||||||
| Article Number: | 565975 | ||||||
| DOI: | 10.3389/fcimb.2020.565975 | ||||||
| Status: | Peer Reviewed | ||||||
| Publication Status: | Published | ||||||
| Access rights to Published version: | Open Access | ||||||
| RIOXX Funder/Project Grant: |
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