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CEP164C regulates flagellum length in stable flagella
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Atkins, Madison, Týč, Jiří, Shafiq, Shahaan, Ahmed, Manu, Bertiaux, Eloïse, De Castro Neto, Artur Leonel, Sunter, Jack, Bastin, Philippe, Dean, Samuel and Vaughan, Sue (2020) CEP164C regulates flagellum length in stable flagella. Journal of Cell Biology, 220 (1). e202001160. doi:10.1083/jcb.202001160 ISSN 0021-9525.
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WRAP-CEP164C-regulates-flagellum-length-stable-flagella-Dean-2020.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (2959Kb) | Preview |
Official URL: https://doi.org/10.1083/jcb.202001160
Abstract
Cilia and flagella are required for cell motility and sensing the external environment and can vary in both length and stability. Stable flagella maintain their length without shortening and lengthening and are proposed to “lock” at the end of growth, but molecular mechanisms for this lock are unknown. We show that CEP164C contributes to the locking mechanism at the base of the flagellum in Trypanosoma brucei. CEP164C localizes to mature basal bodies of fully assembled old flagella, but not to growing new flagella, and basal bodies only acquire CEP164C in the third cell cycle after initial assembly. Depletion of CEP164C leads to dysregulation of flagellum growth, with continued growth of the old flagellum, consistent with defects in a flagellum locking mechanism. Inhibiting cytokinesis results in CEP164C acquisition on the new flagellum once it reaches the old flagellum length. These results provide the first insight into the molecular mechanisms regulating flagella growth in cells that must maintain existing flagella while growing new flagella.
| Item Type: | Journal Article | ||||||||||||||||||||||||
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| Subjects: | Q Science > QH Natural history Q Science > QR Microbiology |
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| Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School | ||||||||||||||||||||||||
| SWORD Depositor: | Library Publications Router | ||||||||||||||||||||||||
| Library of Congress Subject Headings (LCSH): | Flagella (Microbiology) , Cells -- Motility, Cytology | ||||||||||||||||||||||||
| Journal or Publication Title: | Journal of Cell Biology | ||||||||||||||||||||||||
| Publisher: | Rockefeller University Press | ||||||||||||||||||||||||
| ISSN: | 0021-9525 | ||||||||||||||||||||||||
| Official Date: | 9 November 2020 | ||||||||||||||||||||||||
| Dates: |
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| Volume: | 220 | ||||||||||||||||||||||||
| Number: | 1 | ||||||||||||||||||||||||
| Article Number: | e202001160 | ||||||||||||||||||||||||
| DOI: | 10.1083/jcb.202001160 | ||||||||||||||||||||||||
| Status: | Peer Reviewed | ||||||||||||||||||||||||
| Publication Status: | Published | ||||||||||||||||||||||||
| Access rights to Published version: | Open Access (Creative Commons) | ||||||||||||||||||||||||
| Date of first compliant deposit: | 3 November 2022 | ||||||||||||||||||||||||
| Date of first compliant Open Access: | 4 November 2022 | ||||||||||||||||||||||||
| RIOXX Funder/Project Grant: |
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| Is Part Of: | 1 |
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