Skip to content Skip to navigation
University of Warwick
  • Study
  • |
  • Research
  • |
  • Business
  • |
  • Alumni
  • |
  • News
  • |
  • About

University of Warwick
Publications service & WRAP

Highlight your research

  • WRAP
    • Home
    • Search WRAP
    • Browse by Warwick Author
    • Browse WRAP by Year
    • Browse WRAP by Subject
    • Browse WRAP by Department
    • Browse WRAP by Funder
    • Browse Theses by Department
  • Publications Service
    • Home
    • Search Publications Service
    • Browse by Warwick Author
    • Browse Publications service by Year
    • Browse Publications service by Subject
    • Browse Publications service by Department
    • Browse Publications service by Funder
  • Help & Advice
University of Warwick

The Library

  • Login
  • Admin

Translational control of gene expression noise and its relationship to ageing in yeast

Tools
- Tools
+ Tools

de Souza‐Guerreiro, Tailise Carolina , Meng, Xiang, Dacheux, Estelle, Firczuk, Helena and McCarthy, John E. G. (2020) Translational control of gene expression noise and its relationship to ageing in yeast. The FEBS Journal . doi:10.1111/febs.15594 (In Press)

[img]
Preview
PDF
WRAP-Translational-control-gene-expression-noise-relationship-ageing-yeast-2020.pdf - Published Version - Requires a PDF viewer.
Available under License Creative Commons Attribution 4.0.

Download (3596Kb) | Preview
Official URL: https://doi.org/10.1111/febs.15594

Request Changes to record.

Abstract

Gene expression noise influences organism evolution and fitness but is poorly understood. There is increasing evidence that the functional roles of components of the translation machinery influence noise intensity. In addition, modulation of the activities of at least some of these same components affects the replicative lifespan of a broad spectrum of organisms. In a novel comparative approach, we modulate the activities of the translation initiation factors eIFG1 and eIF4G2, both of which are involved in the process of recruiting ribosomal 43S preinitiation complexes to the 5’ end of eukaryotic mRNAs. We show that tagging of the cell wall using a fluorescent dye allows us to follow gene expression noise as different yeast strains progress through successive cycles of replicative ageing. This procedure reveals a relationship between global protein synthesis rate and gene expression noise (cell‐to‐cell heterogeneity), which is accompanied by a parallel correlation between gene expression noise and the replicative age of mother cells. An alternative approach, based on microfluidics, confirms the interdependence between protein synthesis rate, gene expression noise and ageing. We additionally show that it is important to characterize the influence of the design of the microfluidic device on the nutritional state of the cells during such experiments. Analysis of the noise data derived from flow cytometry and fluorescence microscopy measurements indicates that both the intrinsic and the extrinsic noise components increase as a function of ageing.

Item Type: Journal Article
Subjects: Q Science > QH Natural history
Q Science > QK Botany
Q Science > QP Physiology
Divisions: Faculty of Science > Life Sciences (2010- )
SWORD Depositor: Library Publications Router
Library of Congress Subject Headings (LCSH): Messenger RNA , Aging -- Molecular aspects, Gene expression , Yeast fungi -- Aging
Journal or Publication Title: The FEBS Journal
Publisher: Wiley
ISSN: 1742-4658
Official Date: 14 October 2020
Dates:
DateEvent
12 November 2020Updated
14 October 2020Available
7 October 2020UNSPECIFIED
Date of first compliant deposit: 8 December 2020
DOI: 10.1111/febs.15594
Status: Peer Reviewed
Publication Status: In Press
Access rights to Published version: Open Access
RIOXX Funder/Project Grant:
Project/Grant IDRIOXX Funder NameFunder ID
BB/E024181/1 [BBSRC] Biotechnology and Biological Sciences Research Councilhttp://dx.doi.org/10.13039/501100000268
BB/1020535/1[BBSRC] Biotechnology and Biological Sciences Research Councilhttp://dx.doi.org/10.13039/501100000268
BB/1008349/1[BBSRC] Biotechnology and Biological Sciences Research Councilhttp://dx.doi.org/10.13039/501100000268
BB/M017982/1[BBSRC] Biotechnology and Biological Sciences Research Councilhttp://dx.doi.org/10.13039/501100000268
UNSPECIFIED[EPSRC] Engineering and Physical Sciences Research Councilhttp://dx.doi.org/10.13039/501100000266
PhD scholarship Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorhttp://dx.doi.org/10.13039/501100002322
Related URLs:
  • http://creativecommons.org/licenses/by/4...

Request changes or add full text files to a record

Repository staff actions (login required)

View Item View Item

Downloads

Downloads per month over past year

View more statistics

twitter

Email us: wrap@warwick.ac.uk
Contact Details
About Us