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Introducing affinity and selectivity into galectin-targeting nanoparticles with fluorinated glycan ligands
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Richards, Sarah-Jane, Keenan, Tessa, Vendeville, Jean-Baptiste, Wheatley, David E., Chidwick, Harriet, Budhadev, Darshita, Council, Claire E., Webster, Claire S., Ledru, Helene, Baker, Alexander, Walker, Marc, Galan, M. Carmen, Linclau, Bruno, Fascione, Martin A. and Gibson, Matthew I. (2021) Introducing affinity and selectivity into galectin-targeting nanoparticles with fluorinated glycan ligands. Chemical Science . doi:10.1039/D0SC05360K (In Press)
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WRAP-Introducing-affinity-selectivity-galectintargeting-nanoparticles-fluorinated-glycan-Gibson-2020.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution. Download (1035Kb) | Preview |
Official URL: http://dx.doi.org/10.1039/D0SC05360K
Abstract
Galectins are potential biomarkers and therapeutic targets. However, galectins display broad affinity towards β-galactosides meaning glycan-based (nano)biosensors lack the required selectivity and affinity. Using a polymer-stabilized nanoparticle biosensing platform, we herein demonstrate that the specificity of immobilised lacto-N-biose towards galectins can be ‘turned on/off’ by using site-specific glycan fluorination and in some cases reversal of specificity can be achieved. The panel of fluoro-glycans were obtained by a chemoenzymatic approach, exploiting BiGalK and BiGalHexNAcP enzymes from Bifidobacterium infantis which are shown to tolerate fluorinated glycans, introducing structural diversity which would be very laborious by chemical methods alone. These results demonstrate that integrating non-natural, fluorinated glycans into nanomaterials can encode unprecedented selectivity with potential applications in biosensing.
| Item Type: | Journal Article | ||||||||||||||||||||||||||||||||||||
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| Subjects: | Q Science > QD Chemistry Q Science > QP Physiology |
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| Divisions: | Faculty of Science > Chemistry Faculty of Science > Physics Faculty of Medicine > Warwick Medical School |
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| Library of Congress Subject Headings (LCSH): | Lectins , Galactose, Ligands (Biochemistry), Glycosylation | ||||||||||||||||||||||||||||||||||||
| Journal or Publication Title: | Chemical Science | ||||||||||||||||||||||||||||||||||||
| Publisher: | Royal Society of Chemistry | ||||||||||||||||||||||||||||||||||||
| ISSN: | 2041-6520 | ||||||||||||||||||||||||||||||||||||
| Official Date: | 2021 | ||||||||||||||||||||||||||||||||||||
| Dates: |
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| Date of first compliant deposit: | 25 November 2020 | ||||||||||||||||||||||||||||||||||||
| DOI: | 10.1039/D0SC05360K | ||||||||||||||||||||||||||||||||||||
| Status: | Peer Reviewed | ||||||||||||||||||||||||||||||||||||
| Publication Status: | In Press | ||||||||||||||||||||||||||||||||||||
| Access rights to Published version: | Open Access | ||||||||||||||||||||||||||||||||||||
| RIOXX Funder/Project Grant: |
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