The Library
Introducing affinity and selectivity into galectin-targeting nanoparticles with fluorinated glycan ligands
Tools
Richards, Sarah-Jane, Keenan, Tessa, Vendeville, Jean-Baptiste, Wheatley, David E., Chidwick, Harriet, Budhadev, Darshita, Council, Claire E., Webster, Claire S., Ledru, Helene, Baker, Alexander, Walker, Marc, Galan, M. Carmen, Linclau, Bruno, Fascione, Martin A. and Gibson, Matthew I. (2021) Introducing affinity and selectivity into galectin-targeting nanoparticles with fluorinated glycan ligands. Chemical Science, 12 (3). pp. 905-910. doi:10.1039/D0SC05360K ISSN 2041-6520.
|
PDF
WRAP-Introducing-affinity-selectivity-galectintargeting-nanoparticles-fluorinated-glycan-Gibson-2020.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution. Download (1035Kb) | Preview |
Official URL: http://dx.doi.org/10.1039/D0SC05360K
Abstract
Galectins are potential biomarkers and therapeutic targets. However, galectins display broad affinity towards β-galactosides meaning glycan-based (nano)biosensors lack the required selectivity and affinity. Using a polymer-stabilized nanoparticle biosensing platform, we herein demonstrate that the specificity of immobilised lacto-N-biose towards galectins can be ‘turned on/off’ by using site-specific glycan fluorination and in some cases reversal of specificity can be achieved. The panel of fluoro-glycans were obtained by a chemoenzymatic approach, exploiting BiGalK and BiGalHexNAcP enzymes from Bifidobacterium infantis which are shown to tolerate fluorinated glycans, introducing structural diversity which would be very laborious by chemical methods alone. These results demonstrate that integrating non-natural, fluorinated glycans into nanomaterials can encode unprecedented selectivity with potential applications in biosensing.
Item Type: | Journal Article | ||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Subjects: | Q Science > QD Chemistry Q Science > QP Physiology |
||||||||||||||||||||||||||||||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry Faculty of Science, Engineering and Medicine > Science > Physics Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
||||||||||||||||||||||||||||||||||||
Library of Congress Subject Headings (LCSH): | Lectins , Galactose, Ligands (Biochemistry), Glycosylation | ||||||||||||||||||||||||||||||||||||
Journal or Publication Title: | Chemical Science | ||||||||||||||||||||||||||||||||||||
Publisher: | Royal Society of Chemistry | ||||||||||||||||||||||||||||||||||||
ISSN: | 2041-6520 | ||||||||||||||||||||||||||||||||||||
Official Date: | 21 January 2021 | ||||||||||||||||||||||||||||||||||||
Dates: |
|
||||||||||||||||||||||||||||||||||||
Volume: | 12 | ||||||||||||||||||||||||||||||||||||
Number: | 3 | ||||||||||||||||||||||||||||||||||||
Page Range: | pp. 905-910 | ||||||||||||||||||||||||||||||||||||
DOI: | 10.1039/D0SC05360K | ||||||||||||||||||||||||||||||||||||
Status: | Peer Reviewed | ||||||||||||||||||||||||||||||||||||
Publication Status: | Published | ||||||||||||||||||||||||||||||||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||||||||||||||||||||||||||||||||
Date of first compliant deposit: | 25 November 2020 | ||||||||||||||||||||||||||||||||||||
Date of first compliant Open Access: | 25 November 2020 | ||||||||||||||||||||||||||||||||||||
RIOXX Funder/Project Grant: |
|
Request changes or add full text files to a record
Repository staff actions (login required)
View Item |
Downloads
Downloads per month over past year