GenTHREADER: An efficient and reliable protein fold recognition method for genomic sequences
UNSPECIFIED. (1999) GenTHREADER: An efficient and reliable protein fold recognition method for genomic sequences. Journal of Molecular Biology, 287 (4). pp. 797-815. ISSN 0022-2836Full text not available from this repository.
A new protein fold recognition method is described which is both fast and reliable. The method uses a traditional sequence alignment algorithm to generate alignments which are then evaluated by a method derived from threading techniques. As a final step, each threaded model is evaluated by a neural network in order to produce a single measure of confidence in the proposed prediction. The speed of the method, along with its sensitivity and very low false-positive rate makes it ideal for automatically predicting the structure of all the proteins in a translated bacterial genome (proteome). The method has been applied to the genome of Mycoplasma genitalium, and analysis of the results shows that as many as 46% of the proteins derived from the predicted protein coding regions have a significant relationship to a protein of known structure. Ln some cases, however, only one domain of the protein can be predicted, giving a total coverage of 30 % when calculated as a fraction of the number of amino acid residues in the whole proteome. (C) 1999 Academic Press.
|Item Type:||Journal Article|
|Subjects:||Q Science > QD Chemistry|
|Journal or Publication Title:||Journal of Molecular Biology|
|Publisher:||ACADEMIC PRESS LTD|
|Date:||9 April 1999|
|Number of Pages:||19|
|Page Range:||pp. 797-815|
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