The Library
Transcription factor Pit-1 affects transcriptional timing in the dual-promoter human prolactin gene
Tools
Tools
Tools
McNamara, Anne V, Awais, Raheela, Momiji, Hiroshi, Dunham, Lee, Featherstone, Karen, Harper, Claire V, Adamson, Antony A, Semprini, Sabrina, Jones, Nicholas A, Spiller, David G, Mullins, John J, Finkenstädt, Bärbel, Rand, David A., White, Michael R. H. and Davis, Julian R. E. (2021) Transcription factor Pit-1 affects transcriptional timing in the dual-promoter human prolactin gene. Endocrinology, 162 (4). bqaa249. doi:10.1210/endocr/bqaa249 ISSN 0013-7227.
|
PDF
WRAP-Transcription-factor-Pit-1-affects-transcriptional-timing-dual-promoter-human-prolactin-gene-Rand-2021.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (2035Kb) | Preview |
|
![[img]](https://wrap.warwick.ac.uk/style/images/fileicons/application_pdf.png) |
PDF
WRAP-Transcription-factor-Pit-1-affects-transcriptional-timing-dual-promoter-human-prolactin-gene-Rand-2021.pdf - Accepted Version Embargoed item. Restricted access to Repository staff only - Requires a PDF viewer. Download (3488Kb) |
Official URL: http://dx.doi.org/10.1210/endocr/bqaa249
Abstract
Gene transcription occurs in short bursts interspersed with silent periods, and these kinetics can be altered by promoter structure. The effect of alternate promoter architecture on transcription bursting is not known. We studied the human prolactin (hPRL) gene that contains two promoters, a pituitary-specific promoter that requires the transcription factor Pit-1, and displays dramatic transcriptional bursting activity, and an alternate upstream promoter that is active in non-pituitary tissues. We studied large hPRL genomic fragments with luciferase reporters, and used bacterial artificial chromosome (BAC) recombineering to manipulate critical promoter regions. Stochastic switch mathematical modelling of single-cell time-lapse luminescence image data revealed that the Pit-1-dependent promoter showed longer, higher-amplitude transcriptional bursts. Knockdown studies confirmed that the presence of Pit-1 stabilised and prolonged periods of active transcription. Pit-1 therefore plays an active role in establishing the timing of transcription cycles, in addition to its cell-specific functions.
| Item Type: | Journal Article | |||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Subjects: | Q Science > QA Mathematics Q Science > QH Natural history Q Science > QH Natural history > QH426 Genetics Q Science > QP Physiology |
|||||||||||||||||||||
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Mathematics | |||||||||||||||||||||
| Library of Congress Subject Headings (LCSH): | Genetic transformation, Promoters (Genetics), Prolactin genes, Luciferases, Bacterial artificial chromosomes, Stochastic models | |||||||||||||||||||||
| Journal or Publication Title: | Endocrinology | |||||||||||||||||||||
| Publisher: | Endocrine Society | |||||||||||||||||||||
| ISSN: | 0013-7227 | |||||||||||||||||||||
| Official Date: | April 2021 | |||||||||||||||||||||
| Dates: |
|
|||||||||||||||||||||
| Volume: | 162 | |||||||||||||||||||||
| Number: | 4 | |||||||||||||||||||||
| Article Number: | bqaa249 | |||||||||||||||||||||
| DOI: | 10.1210/endocr/bqaa249 | |||||||||||||||||||||
| Status: | Peer Reviewed | |||||||||||||||||||||
| Publication Status: | Published | |||||||||||||||||||||
| Access rights to Published version: | Open Access (Creative Commons) | |||||||||||||||||||||
| Date of first compliant deposit: | 7 January 2021 | |||||||||||||||||||||
| Date of first compliant Open Access: | 11 January 2021 | |||||||||||||||||||||
| RIOXX Funder/Project Grant: |
|
Request changes or add full text files to a record
Repository staff actions (login required)
 |
View Item |
Downloads
Downloads per month over past year
