UNSPECIFIED (1999) Molecular analysis of a novel methanesulfonic acid monooxygenase from the methylotroph Methylosulfonomonas methylovora. JOURNAL OF BACTERIOLOGY, 181 (7). pp. 2244-2251. ISSN 0021-9193

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Abstract

Methylosulfonomonas methylovora M2 is an unusual gram-negative methylotrophic bacterium that can grow on methanesulfonic acid (MSA) as the sole source of carbon and energy, Oxidation of MSA by this bacterium is carried out by a multicomponent MSA monooxygenase (MSAMO). Cloning and sequencing of a 7.5-kbp,sphI fragment of chromosomal DNA revealed four tightly linked genes encoding this no,el monooxygenase, Analysis of the deduced MSAMO polypeptide sequences indicated that the enzyme contains a two-component hydroxylase of the mononuclear-iron-center type, The large subunit of the hydroxylase, MsmA (48 kDa), contains a typical Rieske-type [2Fe-2S] center with an unusual iron-binding motif and, together with the small subunit of the hydroxylase, MsmB (20 kDa), showed a high degree of identity with a number of dioxygenase enzymes. However, the other components of the MSAMO, MsmC, the ferredoxin component, and MsmD, the reductase, more closely resemble those found in other classes of oxygenases. MsmC has a high degree of identity to ferredoxins from toluene and methane monooxygenases, which are enzymes characterized by possessing hydroxylases containing mu-oxo bridge binuclear iron centers. MsmD is a reductase of 38 kDa with a typical chloroplast-like [2Fe-2S] center and conserved flavin adenine dinucleotide- and NAD-binding motifs and is similar to a number of mono- and dioxygenase reductase components. Preliminary analysis of the genes encoding MSAMO from a marine MSA-degrading bacterium, Marinosulfonomonas methylotropha, revealed the presence of msm genes highly related to those found in Methylosulfonomonas, suggesting that MSAMO is a no,el type of oxygenase that may be conserved in all MSA-utilizing bacteria.

Item Type:	Journal Article
Subjects:	Q Science > QR Microbiology
Journal or Publication Title:	JOURNAL OF BACTERIOLOGY
Publisher:	AMER SOC MICROBIOLOGY
ISSN:	0021-9193
Date:	April 1999
Volume:	181
Number:	7
Number of Pages:	8
Page Range:	pp. 2244-2251
Publication Status:	Published
URI:	http://wrap.warwick.ac.uk/id/eprint/14690

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