The Library
MicroRNAs buffer genetic variation at specific temperatures during embryonic development
Tools
Amourda, Christopher, Chong, Jeronica and Saunders, Timothy E. (2018) MicroRNAs buffer genetic variation at specific temperatures during embryonic development. Working Paper. BioRxiv: Cold Spring Harbour. (Unpublished)
Research output not available from this repository.
Request-a-Copy directly from author or use local Library Get it For Me service.
Official URL: http://dx.doi.org/10.1101/444810
Abstract
Successful embryogenesis requires the coordination of developmental events. Perturbations, such as environmental changes, must be buffered to ensure robust development. However, how such buffering occurs is currently unknown in most developmental systems. Here, we demonstrate that seven miRNAs are differentially expressed during Drosophila embryogenesis at varying temperatures within natural physiological ranges. Lack of miR-3-309, -31a, -310c, -980 or -984c causes developmental delays specifically at a given temperature. Detailed analysis on miR-310c and -984c shows that their targets are typically mis-expressed in mutant backgrounds, with phenotypes more pronounced at temperatures where miRNAs show highest expression in wild-type embryos. Our results show that phenotypes may arise at specific temperatures while remaining silent at others, even within typical temperature ranges. Our work uncovers that miRNAs mask genetic variation at specific temperatures to increase embryonic robustness, highlighting another layer of complexity in miRNA expression.
Item Type: | Working or Discussion Paper (Working Paper) | ||||
---|---|---|---|---|---|
Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
||||
Publisher: | Cold Spring Harbour | ||||
Place of Publication: | BioRxiv | ||||
Book Title: | MicroRNAs buffer genetic variation at specific temperatures during embryonic development | ||||
Official Date: | 2018 | ||||
Dates: |
|
||||
DOI: | 10.1101/444810 | ||||
Institution: | University of Warwick | ||||
Publication Status: | Unpublished | ||||
Access rights to Published version: | Open Access (Creative Commons) |
Request changes or add full text files to a record
Repository staff actions (login required)
View Item |