Amourda, Christopher, Chong, Jeronica and Saunders, Timothy E. (2018) MicroRNAs buffer genetic variation at specific temperatures during embryonic development. Working Paper. BioRxiv: Cold Spring Harbour. (Unpublished)

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Abstract

Successful embryogenesis requires the coordination of developmental events. Perturbations, such as environmental changes, must be buffered to ensure robust development. However, how such buffering occurs is currently unknown in most developmental systems. Here, we demonstrate that seven miRNAs are differentially expressed during Drosophila embryogenesis at varying temperatures within natural physiological ranges. Lack of miR-3-309, -31a, -310c, -980 or -984c causes developmental delays specifically at a given temperature. Detailed analysis on miR-310c and -984c shows that their targets are typically mis-expressed in mutant backgrounds, with phenotypes more pronounced at temperatures where miRNAs show highest expression in wild-type embryos. Our results show that phenotypes may arise at specific temperatures while remaining silent at others, even within typical temperature ranges. Our work uncovers that miRNAs mask genetic variation at specific temperatures to increase embryonic robustness, highlighting another layer of complexity in miRNA expression.

Item Type:	Working or Discussion Paper (Working Paper)
Divisions:	Faculty of Medicine > Warwick Medical School > Biomedical Sciences Faculty of Medicine > Warwick Medical School
Publisher:	Cold Spring Harbour
Place of Publication:	BioRxiv
Book Title:	MicroRNAs buffer genetic variation at specific temperatures during embryonic development
Official Date:	2018
Dates:	Date Event 2018 Available
DOI:	10.1101/444810
Institution:	University of Warwick
Publication Status:	Unpublished
Access rights to Published version:	Open Access

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