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Bicoid gradient formation mechanism and dynamics revealed by protein lifetime analysis
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Durrieu, Lucia, Kirrmaier, Daniel, Schneidt, Tatjana, Kats, Ilia, Raghavan, Sarada, Hufnagel, Lars, Saunders, Timothy E. and Knop, Michael (2018) Bicoid gradient formation mechanism and dynamics revealed by protein lifetime analysis. Molecular Systems Biology, 14 (9). e8355. doi:10.15252/msb.20188355
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WRAP-bicoid-gradient-formation-mechanism-dynamics-revealed-protein-lifetime-analysis-Saunders-2018.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (2383Kb) | Preview |
Official URL: http://dx.doi.org/10.15252/msb.20188355
Abstract
Embryogenesis relies on instructions provided by spatially organized signaling molecules known as morphogens. Understanding the principles behind morphogen distribution and how cells interpret locally this information remains a major challenge in developmental biology. Here, we introduce morphogen‐age measurements as a novel approach to test models of morphogen gradient formation. Using a tandem fluorescent timer as a protein age sensor, we find a gradient of increasing age of Bicoid along the anterior–posterior axis in the early Drosophila embryo. Quantitative analysis of the protein age distribution across the embryo reveals that the synthesis–diffusion–degradation model is the most likely model underlying Bicoid gradient formation, and rules out other hypotheses for gradient formation. Moreover, we show that the timer can detect transitions in the dynamics associated with syncytial cellularization. Our results provide new insight into Bicoid gradient formation and demonstrate how morphogen‐age information can complement knowledge about movement, abundance, and distribution, which should be widely applicable to other systems.
| Item Type: | Journal Article | ||||||||||||||||||||||||
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| Subjects: | Q Science > QH Natural history > QH301 Biology Q Science > QL Zoology |
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| Divisions: | Faculty of Medicine > Warwick Medical School > Biomedical Sciences Faculty of Medicine > Warwick Medical School |
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| Library of Congress Subject Headings (LCSH): | Drosophila melanogaster, Embryology, Biological systems -- Research, Developmental biology | ||||||||||||||||||||||||
| Journal or Publication Title: | Molecular Systems Biology | ||||||||||||||||||||||||
| Publisher: | Nature Publishing Group | ||||||||||||||||||||||||
| ISSN: | 1744-4292 | ||||||||||||||||||||||||
| Official Date: | 2018 | ||||||||||||||||||||||||
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| Volume: | 14 | ||||||||||||||||||||||||
| Number: | 9 | ||||||||||||||||||||||||
| Article Number: | e8355 | ||||||||||||||||||||||||
| DOI: | 10.15252/msb.20188355 | ||||||||||||||||||||||||
| Status: | Peer Reviewed | ||||||||||||||||||||||||
| Publication Status: | Published | ||||||||||||||||||||||||
| Access rights to Published version: | Open Access | ||||||||||||||||||||||||
| RIOXX Funder/Project Grant: |
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