Skip to content Skip to navigation
University of Warwick
  • Study
  • |
  • Research
  • |
  • Business
  • |
  • Alumni
  • |
  • News
  • |
  • About

University of Warwick
Publications service & WRAP

Highlight your research

  • WRAP
    • Home
    • Search WRAP
    • Browse by Warwick Author
    • Browse WRAP by Year
    • Browse WRAP by Subject
    • Browse WRAP by Department
    • Browse WRAP by Funder
    • Browse Theses by Department
  • Publications Service
    • Home
    • Search Publications Service
    • Browse by Warwick Author
    • Browse Publications service by Year
    • Browse Publications service by Subject
    • Browse Publications service by Department
    • Browse Publications service by Funder
  • Help & Advice
University of Warwick

The Library

  • Login
  • Admin

Geometric constraints alter cell arrangements within curved epithelial tissues

Tools
- Tools
+ Tools

Rupprecht, Jean-Francois, Ong, Kok Haur, Yin, Jianmin, Huang, Anqi, Dinh, Huy-Hong-Quan, Singh, Anand P., Zhang, Shaobo, Yu, Weimiao, Saunders, Timothy E. and Yap, Alpha (2017) Geometric constraints alter cell arrangements within curved epithelial tissues. Molecular Biology of the Cell, 28 (25). pp. 3582-3594. doi:10.1091/mbc.E17-01-0060

[img]
Preview
PDF
WRAP-geometric-constraints-alter-cell-arrangements-within-curved-epithelial-tissues-Saunders-2017.pdf - Published Version - Requires a PDF viewer.
Available under License Creative Commons Attribution Non-commercial Share Alike.

Download (3469Kb) | Preview
Official URL: https://doi.org/10.1091/mbc.E17-01-0060

Request Changes to record.

Abstract

Organ and tissue formation are complex three-dimensional processes involving cell division, growth, migration, and rearrangement, all of which occur within physically constrained regions. However, analyzing such processes in three dimensions in vivo is challenging. Here, we focus on the process of cellularization in the anterior pole of the early Drosophila embryo to explore how cells compete for space under geometric constraints. Using microfluidics combined with fluorescence microscopy, we extract quantitative information on the three-dimensional epithelial cell morphology. We observed a cellular membrane rearrangement in which cells exchange neighbors along the apical-basal axis. Such apical-to-basal neighbor exchanges were observed more frequently in the anterior pole than in the embryo trunk. Furthermore, cells within the anterior pole skewed toward the trunk along their long axis relative to the embryo surface, with maximum skew on the ventral side. We constructed a vertex model for cells in a curved environment. We could reproduce the observed cellular skew in both wild-type embryos and embryos with distorted morphology. Further, such modeling showed that cell rearrangements were more likely in ellipsoidal, compared with cylindrical, geometry. Overall, we demonstrate that geometric constraints can influence three-dimensional cell morphology and packing within epithelial tissues.

Item Type: Journal Article
Subjects: Q Science > QL Zoology
Q Science > QP Physiology
Divisions: Faculty of Medicine > Warwick Medical School > Biomedical Sciences
Faculty of Medicine > Warwick Medical School
Library of Congress Subject Headings (LCSH): Epithelial cells, Drosophila
Journal or Publication Title: Molecular Biology of the Cell
Publisher: American Society for Cell Biology
ISSN: 1059-1524
Official Date: 1 December 2017
Dates:
DateEvent
1 December 2017Published
4 October 2017Available
27 September 2017Accepted
Date of first compliant deposit: 13 January 2021
Volume: 28
Number: 25
Page Range: pp. 3582-3594
DOI: 10.1091/mbc.E17-01-0060
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Open Access
RIOXX Funder/Project Grant:
Project/Grant IDRIOXX Funder NameFunder ID
2012NRF-NRFF001-094National Research Foundation Singaporehttp://dx.doi.org/10.13039/501100001381
UNSPECIFIEDMechanobiology Institute, Singaporehttp://dx.doi.org/10.13039/501100007672
UNSPECIFIEDInstitute of Molecular and Cell Biologyhttp://dx.doi.org/10.13039/501100007674

Request changes or add full text files to a record

Repository staff actions (login required)

View Item View Item

Downloads

Downloads per month over past year

View more statistics

twitter

Email us: wrap@warwick.ac.uk
Contact Details
About Us