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Geometric constraints alter cell arrangements within curved epithelial tissues
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Rupprecht, Jean-Francois, Ong, Kok Haur, Yin, Jianmin, Huang, Anqi, Dinh, Huy-Hong-Quan, Singh, Anand P., Zhang, Shaobo, Yu, Weimiao, Saunders, Timothy E. and Yap, Alpha (2017) Geometric constraints alter cell arrangements within curved epithelial tissues. Molecular Biology of the Cell, 28 (25). pp. 3582-3594. doi:10.1091/mbc.E17-01-0060
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WRAP-geometric-constraints-alter-cell-arrangements-within-curved-epithelial-tissues-Saunders-2017.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution Non-commercial Share Alike. Download (3469Kb) | Preview |
Official URL: https://doi.org/10.1091/mbc.E17-01-0060
Abstract
Organ and tissue formation are complex three-dimensional processes involving cell division, growth, migration, and rearrangement, all of which occur within physically constrained regions. However, analyzing such processes in three dimensions in vivo is challenging. Here, we focus on the process of cellularization in the anterior pole of the early Drosophila embryo to explore how cells compete for space under geometric constraints. Using microfluidics combined with fluorescence microscopy, we extract quantitative information on the three-dimensional epithelial cell morphology. We observed a cellular membrane rearrangement in which cells exchange neighbors along the apical-basal axis. Such apical-to-basal neighbor exchanges were observed more frequently in the anterior pole than in the embryo trunk. Furthermore, cells within the anterior pole skewed toward the trunk along their long axis relative to the embryo surface, with maximum skew on the ventral side. We constructed a vertex model for cells in a curved environment. We could reproduce the observed cellular skew in both wild-type embryos and embryos with distorted morphology. Further, such modeling showed that cell rearrangements were more likely in ellipsoidal, compared with cylindrical, geometry. Overall, we demonstrate that geometric constraints can influence three-dimensional cell morphology and packing within epithelial tissues.
| Item Type: | Journal Article | ||||||||||||
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| Subjects: | Q Science > QL Zoology Q Science > QP Physiology |
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| Divisions: | Faculty of Medicine > Warwick Medical School > Biomedical Sciences Faculty of Medicine > Warwick Medical School |
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| Library of Congress Subject Headings (LCSH): | Epithelial cells, Drosophila | ||||||||||||
| Journal or Publication Title: | Molecular Biology of the Cell | ||||||||||||
| Publisher: | American Society for Cell Biology | ||||||||||||
| ISSN: | 1059-1524 | ||||||||||||
| Official Date: | 1 December 2017 | ||||||||||||
| Dates: |
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| Date of first compliant deposit: | 13 January 2021 | ||||||||||||
| Volume: | 28 | ||||||||||||
| Number: | 25 | ||||||||||||
| Page Range: | pp. 3582-3594 | ||||||||||||
| DOI: | 10.1091/mbc.E17-01-0060 | ||||||||||||
| Status: | Peer Reviewed | ||||||||||||
| Publication Status: | Published | ||||||||||||
| Access rights to Published version: | Open Access | ||||||||||||
| RIOXX Funder/Project Grant: |
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