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Basolateral protrusion and apical contraction cooperatively drive Drosophila germ-band extension
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Sun, Zijun, Amourda, Christopher, Shagirov, Murat, Hara, Yusuke, Saunders, Timothy E. and Toyama, Yusuke (2017) Basolateral protrusion and apical contraction cooperatively drive Drosophila germ-band extension. Nature Cell Biology, 19 (4). pp. 375-383. doi:10.1038/ncb3497
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Official URL: http://dx.doi.org/10.1038/ncb3497
Abstract
Throughout development, tissues undergo complex morphological changes, resulting from cellular mechanics that evolve over time and in three-dimensional space. During Drosophila germ-band extension (GBE), cell intercalation is the key mechanism for tissue extension1, and the associated apical junction remodelling is driven by polarized myosin-II-dependent contraction2,3,4. However, the contribution of the basolateral cellular mechanics to GBE remains poorly understood. Here, we characterize how cells coordinate their shape from the apical to the basal side during rosette formation, a hallmark of cell intercalation. Basolateral rosette formation is driven by cells mostly located at the dorsal/ventral part of the rosette (D/V cells). These cells exhibit actin-rich wedge-shaped basolateral protrusions and migrate towards each other. Surprisingly, the formation of basolateral rosettes precedes that of the apical rosettes. Basolateral rosette formation is independent of apical contractility, but requires Rac1-dependent protrusive motility. Furthermore, we identified Src42A as a regulator of basolateral rosette formation. Our data show that in addition to apical contraction, active cell migration driven by basolateral protrusions plays a pivotal role in rosette formation and contributes to GBE.
| Item Type: | Journal Article | ||||
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| Divisions: | Faculty of Medicine > Warwick Medical School > Biomedical Sciences Faculty of Medicine > Warwick Medical School |
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| Journal or Publication Title: | Nature Cell Biology | ||||
| Publisher: | Nature Publishing Group | ||||
| ISSN: | 1465-7392 | ||||
| Official Date: | 27 March 2017 | ||||
| Dates: |
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| Volume: | 19 | ||||
| Number: | 4 | ||||
| Page Range: | pp. 375-383 | ||||
| DOI: | 10.1038/ncb3497 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Restricted or Subscription Access |
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