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A simplified and standardized neutralization enzyme immunoassay for the quantification of measles neutralizing antibody
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UNSPECIFIED (1999) A simplified and standardized neutralization enzyme immunoassay for the quantification of measles neutralizing antibody. JOURNAL OF VIROLOGICAL METHODS, 78 (1-2). pp. 209-217.
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Abstract
A simplified and standardized neutralization enzyme immunoassay (Nt-EIA) was developed to detect measles Virus growth in Vero cells and to quantify measles neutralizing antibody. Heat-inactivated sera were diluted serially 4-fold and tested in duplicate. The 50% reduction point (50%RP) of virus growth was calculated using the Reed-Muench formula and the neutralizing antibody titre of test sera was converted into mIU/ml by comparing their 50%RP with that of the international standard serum. The optimal virus input and incubation time were found to be 50-100 plaque forming unit (PFU)/well and 64-72 h, respectively. The simplified Nt-EIA had a good reproducibility with only 3.7-4.2% of duplicate tests having a ratio >4 in an evaluation of intra assay Variation and the coefficients of variance were 2-9% in an evaluation of inter assay variation. In addition, the simplified Nt-EIA had a high sensitivity (98.6%). specificity (100%) and agreement (98.8%) in qualitative comparison with plaque reduction neutralization test (PRNT). In quantitative comparison, the correlation coefficient between Nt-EIA and PRNT was 0.83 without log transformation or 0.77 after log transformation and 90% of 61 positive sera had a ratio <4 between antibody titre tested by the two methods. The simplified Nt-EIA is thus a suitable alternative to the PRNT for the quantification of measles neutralizing antibody. (C) 1999 Elsevier Science B.V. All rights reserved.
| Item Type: | Journal Article | ||||
|---|---|---|---|---|---|
| Subjects: | Q Science > QD Chemistry T Technology > TP Chemical technology Q Science > QR Microbiology > QR355 Virology |
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| Journal or Publication Title: | JOURNAL OF VIROLOGICAL METHODS | ||||
| Publisher: | ELSEVIER SCIENCE BV | ||||
| ISSN: | 0166-0934 | ||||
| Official Date: | March 1999 | ||||
| Dates: |
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| Volume: | 78 | ||||
| Number: | 1-2 | ||||
| Number of Pages: | 9 | ||||
| Page Range: | pp. 209-217 | ||||
| Publication Status: | Published |
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