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New fluorescent auxin probes visualize tissue-specific and subcellular distributions of auxin in Arabidopsis
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Pařízková, Barbora, Žukauskaitė, Asta, Vain, Thomas, Grones, Peter, Raggi, Sara, Kubeš, Martin F., Kieffer, Martin, Doyle, Siamsa M., Strnad, Miroslav, Kepinski, Stefan, Napier, Richard, Doležal, Karel, Robert, Stéphanie and Novák, Ondřej (2021) New fluorescent auxin probes visualize tissue-specific and subcellular distributions of auxin in Arabidopsis. New Phytologist, 230 (2). pp. 535-549. doi:10.1111/nph.17183 ISSN 0028-646X.
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Official URL: https://doi.org/10.1111/nph.17183
Abstract
Summary In a world that will rely increasingly on efficient plant growth for sufficient food, it is important to learn about natural mechanisms of phytohormone action. In this work, the introduction of a fluorophore to an auxin molecule represents a sensitive and non-invasive method to directly visualize auxin localization with high spatiotemporal resolution. The state-of-the-art multidisciplinary approaches of genetic and chemical biology analysis together with live cell imaging, liquid chromatography-mass spectrometry (LC-MS) and surface plasmon resonance (SPR) methods were employed for the characterization of auxin-related biological activity, distribution and stability of the presented compounds in Arabidopsis thaliana. Despite partial metabolization in vivo, these fluorescent auxins display an uneven and dynamic distribution leading to the formation of fluorescence maxima in tissues known to concentrate natural auxin, such as the concave side of the apical hook. Importantly, their distribution is altered in response to different exogenous stimuli in both roots and shoots. Moreover, we characterized the subcellular localization of the fluorescent auxin analogues as being present in the endoplasmic reticulum and endosomes. Our work provides powerful tools to visualize auxin distribution within different plant tissues at cellular or subcellular levels and in response to internal and environmental stimuli during plant development.
| Item Type: | Journal Article | |||||||||||||||||||||||||||||||||||||||
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| Subjects: | Q Science > QC Physics Q Science > QK Botany |
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| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | |||||||||||||||||||||||||||||||||||||||
| Library of Congress Subject Headings (LCSH): | Auxin, Fluorescence, Arabidopsis | |||||||||||||||||||||||||||||||||||||||
| Journal or Publication Title: | New Phytologist | |||||||||||||||||||||||||||||||||||||||
| Publisher: | Wiley-Blackwell Publishing Ltd. | |||||||||||||||||||||||||||||||||||||||
| ISSN: | 0028-646X | |||||||||||||||||||||||||||||||||||||||
| Official Date: | April 2021 | |||||||||||||||||||||||||||||||||||||||
| Dates: |
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| Volume: | 230 | |||||||||||||||||||||||||||||||||||||||
| Number: | 2 | |||||||||||||||||||||||||||||||||||||||
| Page Range: | pp. 535-549 | |||||||||||||||||||||||||||||||||||||||
| DOI: | 10.1111/nph.17183 | |||||||||||||||||||||||||||||||||||||||
| Status: | Peer Reviewed | |||||||||||||||||||||||||||||||||||||||
| Publication Status: | Published | |||||||||||||||||||||||||||||||||||||||
| Reuse Statement (publisher, data, author rights): | "This is the peer reviewed version of the following article: Pařízková, B., Žukauskaitė, A., Vain, T., Grones, P., Raggi, S., Kubeš, M.F., Kieffer, M., Doyle, S.M., Strnad, M., Kepinski, S., Napier, R., Doležal, K., Robert, S. and Novák, O. (2021), New fluorescent auxin probes visualize tissue‐specific and subcellular distributions of auxin in Arabidopsis. New Phytologist. Accepted Author Manuscript. , which has been published in final form at https://doi.org/10.1111/nph.17183. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions." | |||||||||||||||||||||||||||||||||||||||
| Access rights to Published version: | Open Access (Creative Commons) | |||||||||||||||||||||||||||||||||||||||
| Date of first compliant deposit: | 15 January 2021 | |||||||||||||||||||||||||||||||||||||||
| Date of first compliant Open Access: | 18 May 2021 | |||||||||||||||||||||||||||||||||||||||
| RIOXX Funder/Project Grant: |
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