Skip to content Skip to navigation
University of Warwick
  • Study
  • |
  • Research
  • |
  • Business
  • |
  • Alumni
  • |
  • News
  • |
  • About

University of Warwick
Publications service & WRAP

Highlight your research

  • WRAP
    • Home
    • Search WRAP
    • Browse by Warwick Author
    • Browse WRAP by Year
    • Browse WRAP by Subject
    • Browse WRAP by Department
    • Browse WRAP by Funder
    • Browse Theses by Department
  • Publications Service
    • Home
    • Search Publications Service
    • Browse by Warwick Author
    • Browse Publications service by Year
    • Browse Publications service by Subject
    • Browse Publications service by Department
    • Browse Publications service by Funder
  • Help & Advice
University of Warwick

The Library

  • Login
  • Admin

Exometabolomic analysis of decidualizing human endometrial stromal and perivascular cells

Tools
- Tools
+ Tools

Harden, Sarah, Zhou, Jieliang, Gharanei, Seley, Diniz-da-Costa, Maria, Lucas, Emma S., Cui, Liang, Murakami, Keisuke, Fang, Jinling, Chen, Qingfeng, Brosens, Jan J. and Lee, Yie Hou (2021) Exometabolomic analysis of decidualizing human endometrial stromal and perivascular cells. Frontiers, 9 . 626619. doi:10.3389/fcell.2021.626619

[img]
Preview
PDF
WRAP-Exometabolomic-analysis-decidualizing-human-endometrial-stromal-2021.pdf - Published Version - Requires a PDF viewer.
Available under License Creative Commons Attribution 4.0.

Download (3438Kb) | Preview
[img] PDF
WRAP-Exometabolomic-analysis-decidualizing-human-endometrial-stromal-2021.pdf - Accepted Version
Embargoed item. Restricted access to Repository staff only - Requires a PDF viewer.

Download (806Kb)
Official URL: https://doi.org/10.3389/fcell.2021.626619

Request Changes to record.

Abstract

Differentiation of endometrial fibroblasts into specialized decidual cells controls embryo implantation and transforms the cycling endometrium into a semi-permanent, immune-protective matrix that accommodates the placenta throughout pregnancy. This process starts during the midluteal phase of the menstrual cycle with decidual transformation of perivascular cells (PVC) surrounding the terminal spiral arterioles and endometrial stromal cells (EnSC) underlying the luminal epithelium. Decidualization involves extensive cellular reprogramming and acquisition of a secretory phenotype, essential for coordinated placental trophoblast invasion. Secreted metabolites are an emerging class of signalling molecules. Here, we used liquid chromatography-mass spectrometry to characterise the dynamic changes in metabolite secretion (exometabolome) of primary PVC and EnSC decidualized over 8 days. We identified 79 annotated metabolites differentially secreted upon decidualization, including prostaglandin, sphingolipid, and hyaluronic acid metabolites. Secreted metabolites encompassed 21 metabolic pathways, most prominently glycerolipid and pyrimidine metabolism. Although temporal exometabolome changes were comparable between decidualizing PVC and EnSC, 32 metabolites were differentially secreted across the decidualization time-course. Further, targeted metabolomics demonstrated a conspicuous difference in xanthine secretion between decidualized PVC and EnSC. Taken together, our findings indicate that the metabolic footprints generated by different decidual subpopulations encode spatiotemporal information that may be important for optimal embryo implantation.

Item Type: Journal Article
Subjects: Q Science > QH Natural history
Q Science > QP Physiology
Divisions: Faculty of Medicine > Warwick Medical School
Library of Congress Subject Headings (LCSH): Endometrium, Metabolism, Reproduction -- Molecular aspects, Vascular smooth muscle
Journal or Publication Title: Frontiers
Publisher: University of Nebraska Press
ISSN: 0160-9009
Official Date: 28 January 2021
Dates:
DateEvent
28 January 2021Published
11 January 2021Accepted
Volume: 9
Article Number: 626619
DOI: 10.3389/fcell.2021.626619
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Open Access
RIOXX Funder/Project Grant:
Project/Grant IDRIOXX Funder NameFunder ID
NMRC/CG/M003/2017National Research Foundation Singaporehttp://dx.doi.org/10.13039/501100001381
Fellowship : NRF-NRFF2017-03National Research Foundation Singaporehttp://dx.doi.org/10.13039/501100001381
Investigator Award : 212233/Z/18/ZWellcome Trusthttp://dx.doi.org/10.13039/100010269
UNSPECIFIEDUniversity of Warwickhttp://dx.doi.org/10.13039/501100000741
A∗ STAR Research Attachment ProgramAgency for Science, Technology and Researchhttp://dx.doi.org/10.13039/501100001348
Related URLs:
  • Publisher
Open Access Version:
  • bioRxiv

Request changes or add full text files to a record

Repository staff actions (login required)

View Item View Item
twitter

Email us: wrap@warwick.ac.uk
Contact Details
About Us