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The white-rot fungus, Phanerochaete chrysosporium, under combinatorial stress produces variable oil profiles following analysis of secondary metabolites
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Whiteford, Rory, Nurika, Irnia, Schiller, Tara L. and Barker, Guy C. (2021) The white-rot fungus, Phanerochaete chrysosporium, under combinatorial stress produces variable oil profiles following analysis of secondary metabolites. Journal of Applied Microbiology, 131 (3). pp. 1305-1317. doi:10.1111/jam.15013
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Official URL: https://doi.org/10.1111/jam.15013
Abstract
Aims: The effects of combinatorial stress on lipid production in Phanerochaete chrysosporium remains understudied. This species of white‐rot fungi was cultivated on solid‐state media whilst under variable levels of known abiotic and biotic stressors to establish the effect upon fungal oil profiles.
Methods and Results: Environmental stressors induced upon the fungus included: temperature; nutrient limitation; and interspecies competition to assess impact upon oil profiles. Fatty acid type and concentration was determined using analytical methods of Gas Chromatography and Mass Spectrometry. Growth rate under stress was established using High Performance Liquid Chromatography with ergosterol as the biomarker. Fungi grown on solid‐state agar were able to simultaneously produce short and long‐chain fatty acids which appeared to be influenced by nutritional composition as well as temperature. Addition of nitrogen supplements increased the growth rate, but lipid dynamics remained unchanged. Introducing competition‐induced stress had significantly altered the production of certain fatty acids beyond that of the monoculture whilst under nutrient‐limiting conditions. Linoleic acid concentrations, for example, increased from an average of 885 ng/μl at monoculture towards 13820 ng/μl at co‐culture, following 7 days of incubation.
Conclusions: Interspecies competition produced the most notable impact on lipid production for solid‐state media cultivated fungi whilst the addition of nitrogen supplementation presented growth and lipid accumulation to be uncorrelated. Combinatorial stress therefore influences the yield of overall lipid production as well as the number of intermediate fatty acids produced, deriving similar oil profiles to the composition of vegetable and fish oils.
Significance and Impact of Study: Fungal secondary metabolism remains highly sensitive following combinatorial stress. The outcome impacts the research towards optimising fungal oil profiles for biomass and nutrition. Future investigations on fungal stress tolerance mechanisms need to address these environmental factors throughout the experimental design.
| Item Type: | Journal Article | ||||||||
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| Subjects: | Q Science > QK Botany | ||||||||
| Divisions: | Faculty of Science > Life Sciences (2010- ) | ||||||||
| Library of Congress Subject Headings (LCSH): | Phanerochaete, Fungal metabolites, Plant lipids -- Research | ||||||||
| Journal or Publication Title: | Journal of Applied Microbiology | ||||||||
| Publisher: | Wiley-Blackwell Publishing Ltd. | ||||||||
| ISSN: | 1364-5072 | ||||||||
| Official Date: | September 2021 | ||||||||
| Dates: |
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| Volume: | 131 | ||||||||
| Number: | 3 | ||||||||
| Page Range: | pp. 1305-1317 | ||||||||
| DOI: | 10.1111/jam.15013 | ||||||||
| Status: | Peer Reviewed | ||||||||
| Publication Status: | Published | ||||||||
| Publisher Statement: | This is the peer reviewed version of the following article: Whiteford, R., Nurika, I., Schiller, T. and Barker, G. (2021), The white‐rot fungus, Phanerochaete chrysosporium, under combinatorial stress produces variable oil profiles following analysis of secondary metabolites. Journal of Applied Microbiology. Accepted Author Manuscript. , which has been published in final form at https://doi.org/10.1111/jam.15013. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. | ||||||||
| Access rights to Published version: | Open Access | ||||||||
| RIOXX Funder/Project Grant: |
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