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The application of scanning ion conductance microscopy to dental erosion and complex biological systems
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Jones, Bryn (2019) The application of scanning ion conductance microscopy to dental erosion and complex biological systems. PhD thesis, University of Warwick.
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WRAP_Theses_Jones_B_2019.pdf - Submitted Version - Requires a PDF viewer. Download (10Mb) | Preview |
Official URL: http://webcat.warwick.ac.uk/record=b3491582~S15
Abstract
This thesis catalogues further advancement of scanning ion conductance microscopy (SICM) as a multi-functional imaging technique. Through manipulation of the bias applied and interpretation of the modulated feedback the SICM nanopipette is utilised to measure the susceptibility of dental hard tissues to acid dissolution. By delivering protons to enamel surfaces and measuring the depth of the etch pits created in real time, a platform is developed for assessing the resistance of a surface to acid attack. The technique is then utilised to assess the erosive and protective properties of enamel supplements and antagonists. The work cumulates in a multi-step, multi-sample study to investigate sample variance, repeatability and to draw conclusions on the erosive potential of citric acid and the protective benefits of fluoride.
Work is also done on the expansion of SICM surface charge mapping. This technique, developed at Warwick, uses the SICM probe to measure the local ion concentration at surface interfaces. Here, it is validated on more complex systems and its potential for quantifying surface heterogeneities is explored. First, dental enamel and dentine are studied and surface charge anomalies in their microstructures explored and quantified. Secondly the surface charge is explored as an indicator of erosive damage to enamel surfaces. Surface charge changes are observed from mild dietary acid exposure and areas of increased susceptibility are identified on the enamel surface. Finally, the surface charge is examined as a novel indicator of repair through remineralisation of dental enamel.
Surface charge mapping is then used to investigate the charge discrepancies between the cell wall structures of live bacteria. The differences between gram-negative and gram-positive bacterial strains are assessed. To achieve this, novel bacterial adherence techniques were developed to immobilize but not denature the living cells. Advancements are made in the mathematical modelling of the system to explain the unexpectedly large charge values obtained for gram-positive bacteria.
The work sets out to further establish SICM as a premier technique for surface characterisation and multifunctional imaging of biological substrates
Item Type: | Thesis (PhD) | ||||
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Subjects: | Q Science > QD Chemistry Q Science > QH Natural history Q Science > QR Microbiology |
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Library of Congress Subject Headings (LCSH): | Scanning probe microscopy, Ion channels, Gram-negative bacteria, Gram-positive bacteria | ||||
Official Date: | September 2019 | ||||
Dates: |
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Institution: | University of Warwick | ||||
Theses Department: | Department of Chemistry | ||||
Thesis Type: | PhD | ||||
Publication Status: | Unpublished | ||||
Supervisor(s)/Advisor: | Unwin, Patrick R. | ||||
Format of File: | |||||
Extent: | xv, 226 leaves : colour illustrations | ||||
Language: | eng |
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