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Ageing modulates human dermal fibroblast contractility : quantification using nano-biomechanical testing

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Yu, Zhuonan, Smith, Matthew J., Siow, Richard C. M. and Liu, Kuo-Kang (2021) Ageing modulates human dermal fibroblast contractility : quantification using nano-biomechanical testing. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1868 (5). 118972. doi:10.1016/j.bbamcr.2021.118972

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Official URL: http://dx.doi.org/10.1016/j.bbamcr.2021.118972

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Abstract

Dermal fibroblasts play a key role in maintaining homoeostasis and functionality of the skin. Their contractility plays a role in changes observed during ageing, especially in processes such as wound healing, inflammation, wrinkling and scar tissue formation as well as structural changes on extracellular matrix. Although alternations in skin physiology and morphology have been previously described, there remains a paucity of information about the influence of chronological ageing on dermal fibroblast contractility. In this study, we applied a novel nano-biomechanical technique on cell-embedded collagen hydrogels in combination with mathematical modelling and numerical simulation to measure contraction forces of normal human dermal fibroblasts (NHDF). We achieved quantitative differentiation of the contractility of cells derived from ‘young’ (< 30 years old) and ‘aged’ (> 60 years old) donors. Transforming growth factor β1 (TGF-β1) was used to stimulate the fibroblasts to assess their contractile potential. NHDF from aged donors exhibited a greater basal contractile force, while in contrast, NHDF from young donors have shown a significantly larger contractile force in response to TGF-β1 treatment. These findings validate our nano-biomechanical measurement technique and provide new insights for considering NHDF contractility in regenerative medicine and as a biomarker of dermal ageing processes.

Item Type: Journal Article
Subjects: Q Science > QH Natural history
Q Science > QP Physiology
Divisions: Faculty of Science, Engineering and Medicine > Engineering > Engineering
Library of Congress Subject Headings (LCSH): Cells -- Mechanical properties, Fibroblasts -- Adhesion, Fibroblast growth factors , Aging , Skin -- Physiology
Journal or Publication Title: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research
Publisher: Elsevier
ISSN: 0167-4889
Official Date: April 2021
Dates:
DateEvent
April 2021Published
27 January 2021Available
24 January 2021Accepted
Volume: 1868
Number: 5
Article Number: 118972
DOI: 10.1016/j.bbamcr.2021.118972
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Open Access
RIOXX Funder/Project Grant:
Project/Grant IDRIOXX Funder NameFunder ID
UNSPECIFIEDKings College Londonhttp://dx.doi.org/10.13039/501100000764

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