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Long-range control of tissue specific gene expression in Arabidopsis
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Hussain, Nosheed (2019) Long-range control of tissue specific gene expression in Arabidopsis. PhD thesis, University of Warwick.
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Official URL: http://webcat.warwick.ac.uk/record=b3491683~S15
Abstract
The ability of plants to cope with a variety of environmental stresses depends on precise modulation of gene expression. Expression of stress related genes, usually, is under the control of long-range regulatory elements. In order to understand how gene regulation is modulated by these elements, it is important to identify distal regulatory regions and characterise them. One way to identify distal regulatory elements is to measure chromatin accessibility. We have devised a unique in vivo system (iNOMe-seq), to simultaneously measure chromatin accessibility, nucleosome occupancy and methylation profile. Our method outperforms current chromatin accessibility methods in identification of important genomic features with great accuracy in plants. Using our chromatin accessibility data, we identified potential distal regulatory regions (DREs) and a study was carried out to investigate their role in gene regulation by targeted epigenetic and genetic modifications at these regions. Our data shows that hypermethylation of distal regions leads to the down-regulation of gene expression and the phenotypic changes observed after DRE hypermethylation /CRISPR deletion were related to those seen in the mutants of their target genes. Our study concludes that the gene expression can be modulated by modifying their regulatory regions and this approach can be used to modify important crop traits to improve food security.
Item Type: | Thesis (PhD) | ||||
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Subjects: | Q Science > QK Botany S Agriculture > SB Plant culture |
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Library of Congress Subject Headings (LCSH): | Plant genetic regulation, Transgenic plants, Crops -- Genetic engineering | ||||
Official Date: | September 2019 | ||||
Dates: |
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Institution: | University of Warwick | ||||
Theses Department: | School of Life Sciences | ||||
Thesis Type: | PhD | ||||
Publication Status: | Unpublished | ||||
Supervisor(s)/Advisor: | Gutierrez-Marcos, José F. | ||||
Format of File: | |||||
Extent: | xv, 159 leaves : illustrations (chiefly colour) | ||||
Language: | eng |
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