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A nuclear localization signal targets tail-anchored membrane proteins to the inner nuclear envelope in plants
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Groves, Norman R., McKenna, Joseph F., Evans, David E., Graumann, Katja and Meier, Iris (2019) A nuclear localization signal targets tail-anchored membrane proteins to the inner nuclear envelope in plants. Journal of Cell Science, 132 (7). jcs226134. doi:10.1242/jcs.226134 ISSN 0021-9533.
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Official URL: http://dx.doi.org/10.1242/jcs.226134
Abstract
Protein targeting to the inner nuclear membrane (INM) is one of the least understood protein targeting pathways. INM proteins are important for chromatin organization, nuclear morphology and movement, and meiosis, and have been implicated in human diseases. In opisthokonts, one mechanism for INM targeting is transport factor-mediated trafficking, in which nuclear localization signals (NLSs) function in nuclear import of transmembrane proteins. To explore whether this pathway exists in plants, we fused the SV40 NLS to a plant ER tail-anchored protein and showed that the GFP-tagged fusion protein was significantly enriched at the nuclear envelope (NE) of leaf epidermal cells. Airyscan subdiffraction limited confocal microscopy showed that this protein displays a localization consistent with an INM protein. Nine different monopartite and bipartite NLSs from plants and opisthokonts, fused to a chimeric tail-anchored membrane protein, were all sufficient for NE enrichment, and both monopartite and bipartite NLSs were sufficient for trafficking to the INM. Tolerance for different linker lengths and protein conformations suggests that INM trafficking rules might differ from those in opisthokonts. The INM proteins developed here can be used to target new functionalities to the plant nuclear periphery.
Item Type: | Journal Article | ||||||
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||
Journal or Publication Title: | Journal of Cell Science | ||||||
Publisher: | The Company of Biologists Ltd. | ||||||
ISSN: | 0021-9533 | ||||||
Official Date: | 3 April 2019 | ||||||
Dates: |
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Volume: | 132 | ||||||
Number: | 7 | ||||||
Article Number: | jcs226134 | ||||||
DOI: | 10.1242/jcs.226134 | ||||||
Status: | Peer Reviewed | ||||||
Publication Status: | Published | ||||||
Access rights to Published version: | Restricted or Subscription Access |
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