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Identification and characterization of genes encoding the nuclear envelope LINC complex in the monocot species Zea mays
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Gumber, Hardeep K., McKenna, Joseph F., Estrada, Amado L., Tolmie, Andrea F., Graumann, Katja and Bass, Hank W. (2019) Identification and characterization of genes encoding the nuclear envelope LINC complex in the monocot species Zea mays. Journal of Cell Science, 132 (3). jcs221390. doi:10.1242/jcs.221390 ISSN 0021-9533.
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Official URL: http://dx.doi.org/10.1242/jcs.221390
Abstract
The linker of nucleoskeleton to cytoskeleton (LINC) complex is an essential multi-protein structure spanning the nuclear envelope. It connects the cytoplasm to the nucleoplasm, functions to maintain nuclear shape and architecture and regulates chromosome dynamics during cell division. Knowledge of LINC complex composition and function in the plant kingdom is primarily limited to Arabidopsis, but critically missing from the evolutionarily distant monocots, which include grasses, the most important agronomic crops worldwide. To fill this knowledge gap, we identified and characterized 22 maize genes, including a new grass-specific KASH gene family. By using bioinformatic, biochemical and cell biological approaches, we provide evidence that representative KASH candidates localize to the nuclear periphery and interact with Zea mays (Zm)SUN2 in vivo. FRAP experiments using domain deletion constructs verified that this SUN–KASH interaction was dependent on the SUN but not the coiled-coil domain of ZmSUN2. A summary working model is proposed for the entire maize LINC complex encoded by conserved and divergent gene families. These findings expand our knowledge of the plant nuclear envelope in a model grass species, with implications for both basic and applied cellular research.
Item Type: | Journal Article | ||||||
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||
Journal or Publication Title: | Journal of Cell Science | ||||||
Publisher: | The Company of Biologists Ltd. | ||||||
ISSN: | 0021-9533 | ||||||
Official Date: | 11 February 2019 | ||||||
Dates: |
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Volume: | 132 | ||||||
Number: | 3 | ||||||
Article Number: | jcs221390 | ||||||
DOI: | 10.1242/jcs.221390 | ||||||
Status: | Peer Reviewed | ||||||
Publication Status: | Published | ||||||
Access rights to Published version: | Restricted or Subscription Access |
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