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Oxidation of trimethylamine to trimethylamine N-oxide facilitates high hydrostatic pressure tolerance in a generalist bacterial lineage

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Qin, Qi-Long, Wang, Zhi-Bin, Su, Hai-Nan, Chen, Xiu-Lan, Miao, Jie, Wang, Xiu-Juan, Li, Chun-Yang, Zhang, Xi-Ying, Li, Ping-Yi, Wang, Min, Fang, Jiasong, Lidbury, Ian, Zhang, Weipeng, Zhang, Xiao-Hua, Yang, Gui-Peng, Chen, Yin and Zhang, Yu-Zhong (2021) Oxidation of trimethylamine to trimethylamine N-oxide facilitates high hydrostatic pressure tolerance in a generalist bacterial lineage. Science Advances, 7 (13). eabf9941. doi:10.1126/sciadv.abf9941

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Official URL: http://dx.doi.org/10.1126/sciadv.abf9941

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Abstract

High hydrostatic pressure (HHP) is a characteristic environmental factor of the deep ocean. However, it remains unclear how piezotolerant bacteria adapt to HHP. Here, we identify a two-step metabolic pathway to cope with HHP stress in a piezotolerant bacterium. Myroides profundi D25T, obtained from a deep-sea sediment, can take up trimethylamine (TMA) through a previously unidentified TMA transporter, TmaT, and oxidize intracellular TMA into trimethylamine N-oxide (TMAO) by a TMA monooxygenase, MpTmm. The produced TMAO is accumulated in the cell, functioning as a piezolyte, improving both growth and survival at HHP. The function of the TmaT-MpTmm pathway was further confirmed by introducing it into Escherichia coli and Bacillus subtilis. Encoded TmaT-like and MpTmm-like sequences extensively exist in marine metagenomes, and other marine Bacteroidetes bacteria containing genes encoding TmaT-like and MpTmm-like proteins also have improved HHP tolerance in the presence of TMA, implying the universality of this HHP tolerance strategy in marine Bacteroidetes.

Item Type: Journal Article
Subjects: Q Science > QR Microbiology
Divisions: Faculty of Science > Life Sciences (2010- )
Library of Congress Subject Headings (LCSH): Seawater -- Microbiology, Hydrostatic pressure, Oxidation, Trimethylamine, Marine microbiology
Journal or Publication Title: Science Advances
Publisher: American Association for the Advancement of Science
ISSN: 2375-2548
Official Date: 26 March 2021
Dates:
DateEvent
26 March 2021Published
8 January 2021Accepted
Date of first compliant deposit: 10 February 2021
Volume: 7
Number: 13
Article Number: eabf9941
DOI: 10.1126/sciadv.abf9941
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Open Access
RIOXX Funder/Project Grant:
Project/Grant IDRIOXX Funder NameFunder ID
31870101[NSFC] National Natural Science Foundation of Chinahttp://dx.doi.org/10.13039/501100001809
U1706207[NSFC] National Natural Science Foundation of Chinahttp://dx.doi.org/10.13039/501100001809
31630012[NSFC] National Natural Science Foundation of Chinahttp://dx.doi.org/10.13039/501100001809
91851205[NSFC] National Natural Science Foundation of Chinahttp://dx.doi.org/10.13039/501100001809
2018YFC1406700National Science and Technology Infrastructure Program UNSPECIFIED
tspd20181203Shandong Universityhttp://dx.doi.org/10.13039/100009108
2016WLJH36Shandong Universityhttp://dx.doi.org/10.13039/100009108
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