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Oxidation of trimethylamine to trimethylamine N-oxide facilitates high hydrostatic pressure tolerance in a generalist bacterial lineage
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Qin, Qi-Long, Wang, Zhi-Bin, Su, Hai-Nan, Chen, Xiu-Lan, Miao, Jie, Wang, Xiu-Juan, Li, Chun-Yang, Zhang, Xi-Ying, Li, Ping-Yi, Wang, Min, Fang, Jiasong, Lidbury, Ian, Zhang, Weipeng, Zhang, Xiao-Hua, Yang, Gui-Peng, Chen, Yin and Zhang, Yu-Zhong (2021) Oxidation of trimethylamine to trimethylamine N-oxide facilitates high hydrostatic pressure tolerance in a generalist bacterial lineage. Science Advances, 7 (13). eabf9941. doi:10.1126/sciadv.abf9941
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Official URL: http://dx.doi.org/10.1126/sciadv.abf9941
Abstract
High hydrostatic pressure (HHP) is a characteristic environmental factor of the deep ocean. However, it remains unclear how piezotolerant bacteria adapt to HHP. Here, we identify a two-step metabolic pathway to cope with HHP stress in a piezotolerant bacterium. Myroides profundi D25T, obtained from a deep-sea sediment, can take up trimethylamine (TMA) through a previously unidentified TMA transporter, TmaT, and oxidize intracellular TMA into trimethylamine N-oxide (TMAO) by a TMA monooxygenase, MpTmm. The produced TMAO is accumulated in the cell, functioning as a piezolyte, improving both growth and survival at HHP. The function of the TmaT-MpTmm pathway was further confirmed by introducing it into Escherichia coli and Bacillus subtilis. Encoded TmaT-like and MpTmm-like sequences extensively exist in marine metagenomes, and other marine Bacteroidetes bacteria containing genes encoding TmaT-like and MpTmm-like proteins also have improved HHP tolerance in the presence of TMA, implying the universality of this HHP tolerance strategy in marine Bacteroidetes.
| Item Type: | Journal Article | ||||||||||||||||||||||||
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| Subjects: | Q Science > QR Microbiology | ||||||||||||||||||||||||
| Divisions: | Faculty of Science > Life Sciences (2010- ) | ||||||||||||||||||||||||
| Library of Congress Subject Headings (LCSH): | Seawater -- Microbiology, Hydrostatic pressure, Oxidation, Trimethylamine, Marine microbiology | ||||||||||||||||||||||||
| Journal or Publication Title: | Science Advances | ||||||||||||||||||||||||
| Publisher: | American Association for the Advancement of Science | ||||||||||||||||||||||||
| ISSN: | 2375-2548 | ||||||||||||||||||||||||
| Official Date: | 26 March 2021 | ||||||||||||||||||||||||
| Dates: |
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| Volume: | 7 | ||||||||||||||||||||||||
| Number: | 13 | ||||||||||||||||||||||||
| Article Number: | eabf9941 | ||||||||||||||||||||||||
| DOI: | 10.1126/sciadv.abf9941 | ||||||||||||||||||||||||
| Status: | Peer Reviewed | ||||||||||||||||||||||||
| Publication Status: | Published | ||||||||||||||||||||||||
| Access rights to Published version: | Open Access | ||||||||||||||||||||||||
| RIOXX Funder/Project Grant: |
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