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A novel class of sulfur-containing aminolipids widespread in marine roseobacters
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Smith, Alastair F., Silvano, Eleonora, Päuker, Orsola, Guillonneau , Richard, Quareshy, Mussa, Murphy, Andrew, Mausz, Michaela A., Stirrup, Rachel, Rihtman, Branko, Aguilo Ferretjans, Maria, Brandsma, Joost, Petersen, Jörn, Scanlan, David J. and Chen, Yin (2021) A novel class of sulfur-containing aminolipids widespread in marine roseobacters. ISME Journal, 15 . pp. 2440-2453. doi:10.1038/s41396-021-00933-x
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Official URL: https://doi.org/10.1038/s41396-021-00933-x
Abstract
Marine roseobacter group bacteria are numerically abundant and ecologically important players in ocean ecosystems. These bacteria are capable of modifying their membrane lipid composition in response to environmental change. Remarkably, a variety of lipids are produced in these bacteria, including phosphorus-containing glycerophospholipids and several amino acid-containing aminolipids such as ornithine lipids and glutamine lipids. Here, we present the identification and characterization of a novel sulfur-containing aminolipid (SAL) in roseobacters. Using high resolution accurate mass spectrometry, a SAL was found in the lipid extract of Ruegeria pomeroyi DSS-3 and Phaeobacter inhibens DSM 17395. Using comparative genomics, transposon mutagenesis and targeted gene knockout, we identified a gene encoding a putative lyso-lipid acyltransferase, designated salA, which is essential for the biosynthesis of this SAL. Multiple sequence analysis and structural modeling suggest that SalA is a novel member of the lysophosphatidic acid acyltransferase (LPAAT) family, the prototype of which is the PlsC acyltransferase responsible for the biosynthesis of the phospholipid phosphatidic acid. SAL appears to play a key role in biofilm formation in roseobacters. salA is widely distributed in Tara Oceans metagenomes and actively expressed in Tara Oceans metatranscriptomes. Our results raise the importance of sulfur-containing membrane aminolipids in marine bacteria.
| Item Type: | Journal Article | ||||||||||||
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| Subjects: | Q Science > QR Microbiology | ||||||||||||
| Divisions: | Faculty of Science > Life Sciences (2010- ) | ||||||||||||
| Library of Congress Subject Headings (LCSH): | Microbial lipids, Lipids, Bacteria , Marine microbiology | ||||||||||||
| Journal or Publication Title: | ISME Journal | ||||||||||||
| Publisher: | Nature Publishing Group | ||||||||||||
| ISSN: | 1751-7362 | ||||||||||||
| Official Date: | August 2021 | ||||||||||||
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| Volume: | 15 | ||||||||||||
| Page Range: | pp. 2440-2453 | ||||||||||||
| DOI: | 10.1038/s41396-021-00933-x | ||||||||||||
| Status: | Peer Reviewed | ||||||||||||
| Publication Status: | Published | ||||||||||||
| Access rights to Published version: | Open Access | ||||||||||||
| RIOXX Funder/Project Grant: |
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