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Chemical probes reveal the timing of early chlorination in vancomycin biosynthesis
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Leng, Daniel J., Greule, Anja, Cryle, Max J. and Tosin, Manuela (2021) Chemical probes reveal the timing of early chlorination in vancomycin biosynthesis. Chemical Communications, 57 (18). pp. 2293-2296. doi:10.1039/D0CC07421G
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WRAP-chemical-probes-reveal-timing-early-chlorination-vancomycin-biosynthesis-Tosin-2021.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (2365Kb) | Preview |
Official URL: http://dx.doi.org/10.1039/D0CC07421G
Abstract
Glycopeptides such as vancomycin are antibiotics of last resort whose biosynthetic pathways still hold undefined details. Chemical probes were used to capture biosynthetic intermediates generated in the nonribosomal peptide formation of vancomycin in vivo. The putative intercepted intermediates were characterised via HR-LC-MS2. These species provided insights into the timing of the first chlorination of the peptide backbone by the halogenase VhaA: this holds significant interest for enzyme engineering towards the making of novel glycopeptides.
| Item Type: | Journal Article | ||||||||||||
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| Subjects: | Q Science > QP Physiology Q Science > QR Microbiology |
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| Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | ||||||||||||
| Library of Congress Subject Headings (LCSH): | Vancomycin, Vancomycin resistance , Peptides -- Synthesis, Glycopeptides | ||||||||||||
| Journal or Publication Title: | Chemical Communications | ||||||||||||
| Publisher: | Royal Society of Chemistry | ||||||||||||
| ISSN: | 1359-7345 | ||||||||||||
| Official Date: | 2021 | ||||||||||||
| Dates: |
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| Volume: | 57 | ||||||||||||
| Number: | 18 | ||||||||||||
| Page Range: | pp. 2293-2296 | ||||||||||||
| DOI: | 10.1039/D0CC07421G | ||||||||||||
| Status: | Peer Reviewed | ||||||||||||
| Publication Status: | Published | ||||||||||||
| Access rights to Published version: | Open Access | ||||||||||||
| RIOXX Funder/Project Grant: |
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