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Examining cell-cell interactions in the kidney using AFM single-cell force spectroscopy
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Siamantouras, Eleftherios, Hills, Claire E., Liu, Kuo-Kang and Squires, Paul E. (2020) Examining cell-cell interactions in the kidney using AFM single-cell force spectroscopy. In: Gnudi, Luigi and Long, David A., (eds.) Diabetic Nephropathy : Methods & Protocols. Methods in Molecular Biology (2067). Springer Nature, pp. 189-201. ISBN 9781493998401
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Official URL: https://doi.org/10.1007/978-1-4939-9841-8_14
Abstract
The ability of individual cells to synchronize activity is a basic feature of efficient and appropriate tissue function. Central to this is the physicochemical binding between cells through multiprotein complexes that functionally mediate adhesion. Importantly, the direct connection of physical properties and intercellular signaling is of great importance to certain pathologies including diabetes. Atomic force microscopy (AFM) single-cell force spectroscopy (SCFS) is a high-resolution technique that provides a statistically reliable measurement of the minute forces involved in cell tethering and membrane dynamics. Detection of altered nanoscale forces underlying the loss of adhesion in early tubular injury is pivotal for the development of novel therapeutic strategies for diabetic nephropathy. Here we describe the step-by-step use of an integrated AFM-SCFS system designed to measure functional force-displacement in separating renal tubular epithelial cells. Parameters such as unbinding forces, detachment energy, and distance to complete separation can be obtained from force-displacement (F-d) curves and are critical in assessing how physical changes of cellular adhesion contribute to cell contact, coupling, and communication in the diabetic kidney.
Item Type: | Book Item | ||||||
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Subjects: | R Medicine > R Medicine (General) | ||||||
Divisions: | Faculty of Science, Engineering and Medicine > Engineering > Engineering | ||||||
Series Name: | Methods in Molecular Biology | ||||||
Publisher: | Springer Nature | ||||||
ISBN: | 9781493998401 | ||||||
Book Title: | Diabetic Nephropathy : Methods & Protocols | ||||||
Editor: | Gnudi, Luigi and Long, David A. | ||||||
Official Date: | 2020 | ||||||
Dates: |
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Number: | 2067 | ||||||
Page Range: | pp. 189-201 | ||||||
DOI: | 10.1007/978-1-4939-9841-8_14 | ||||||
Status: | Peer Reviewed | ||||||
Publication Status: | Published | ||||||
Access rights to Published version: | Restricted or Subscription Access | ||||||
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