A case of failed oocyte maturation in vivo and in vitro
UNSPECIFIED. (1999) A case of failed oocyte maturation in vivo and in vitro. FERTILITY AND STERILITY, 71 (3). pp. 567-570. ISSN 0015-0282Full text not available from this repository.
Objective: To study a couple for whom all oocytes failed to mature during three attempts at IVF/ICSI treatment. Our aims were (a) to promote oocyte maturation in vitro and (b) to identify the stage of maturation arrest.
Design: Case report.
Setting: Infertility treatment center with academic research links.
Patient(s): Couple with unexplained infertility.
Intervention(s): Exogenous gonadotropin stimulation culminating in the retrieval of right oocyte-cumulus complexes that were exposed to gonadotropins for 24 hours in vitro.
Main Outcome Measure(s): Observation of oocyte cumulus complexes, measurements of oocyte diameter, oocyte chromatin staining with Hoechst 33258,
Result(s): The cumulus complexes varied in appearance and expanded in vitro, but yielded immature oocytes. Germinal vesicle breakdown occurred in all oocytes;and chromatin was consistent with arrest at entry into M-phase. The oocytes were of a size normally competent to mature.
Conclusion(s): Infertility in this couple was attributed to the failure of oocytes to enter M-phase. This was not overcome by exposure to gonadotropins in vitro nor associated with incompetence due to small oocyte size. (Fertil Steril(R) 1999;71.567-70. (C) 1999 by American Society for Reproductive Medicine.)
|Item Type:||Journal Article|
|Subjects:||R Medicine > RG Gynecology and obstetrics|
|Journal or Publication Title:||FERTILITY AND STERILITY|
|Publisher:||ELSEVIER SCIENCE INC|
|Official Date:||March 1999|
|Number of Pages:||4|
|Page Range:||pp. 567-570|
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