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Analysis of biocompatible polymers and cyclic peptide-polymer conjugates by one- and two-dimensional Fourier transform ion cyclotron resonance mass spectrometry
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Morgan, Tomos E. (2020) Analysis of biocompatible polymers and cyclic peptide-polymer conjugates by one- and two-dimensional Fourier transform ion cyclotron resonance mass spectrometry. PhD thesis, University of Warwick.
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WRAP_Theses_Morgan_2020.pdf - Submitted Version - Requires a PDF viewer. Download (14Mb) | Preview |
Official URL: http://webcat.warwick.ac.uk/record=b3492695~S15
Abstract
The work presented in this thesis focusses on the analysis of biocompatible polymers and cyclic peptide-polymer conjugates with the use of Fourier transform ion cyclotron resonance mass spectrometry. The analysis of these polymeric species is carried out extensively by their fragmentation to understand copolymer sequences and end groups.
The thesis contributes a significant number of novel methods and observations relating to the mass spectrometry analysis of polymers. Modified Kendrick mass defect analysis (chapter 2) is used extensively in all of the chapters as a method to simplify the data de novo. Electron capture dissociation methods are shown to be able to characterise polyacrylamides and polyoxazolines. The corresponding studies shows a novel dissociation mechanism for polyacrylamides (chapter 4) and previously unseen hydrolysis properties of polyoxazolines (chapter 5).
Novel ultraviolet dissociation methods produced complete coverage of the polyoxazoline and polyacrylamide polymers used (chapter 6). All of this culminates in the analysis of complex cyclic peptide-polymer conjugates (chapter 7) shows how a suite of mass spectrometry methods can be bought together to further the analysis of these complex polymeric species.
Finally, the final chapter is based on the analysis of polymeric species using two-dimensional mass spectrometry. The ability to analyse multiple polymer species by tandem mass spectrometry, in one experiment, without the need to isolate precursor species, is an incredibly powerful tool within polymer analysis.
Item Type: | Thesis (PhD) | ||||
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Subjects: | Q Science > QD Chemistry | ||||
Library of Congress Subject Headings (LCSH): | Polymers -- Analysis, Cyclic peptides -- Analysis, Ion cyclotron resonance spectrometry | ||||
Official Date: | March 2020 | ||||
Dates: |
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Institution: | University of Warwick | ||||
Theses Department: | Department of Chemistry | ||||
Thesis Type: | PhD | ||||
Publication Status: | Unpublished | ||||
Supervisor(s)/Advisor: | O'Connor, Peter B. ; Perrier, Sébastien ; Bristow, Tony | ||||
Format of File: | |||||
Extent: | xxxii, 371 leaves : illustrations (some colour) | ||||
Language: | eng |
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