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Proteomics insights into the Burkholderia cenocepacia phosphorus stress response
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Shropshire, Holly, Jones, Rebekah A., Aguilo‐Ferretjans, María M., Scanlan, David J. and Chen, Yin (2021) Proteomics insights into the Burkholderia cenocepacia phosphorus stress response. Environmental Microbiology, 23 (9). pp. 5069-5086. doi:10.1111/1462-2920.15451 ISSN 1462-2912.
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Official URL: https://doi.org/10.1111/1462-2920.15451
Abstract
The Burkholderia cepacia complex is a group of Burkholderia species that are opportunistic pathogens causing high mortality rates in patients with cystic fibrosis. An environmental stress often encountered by these soil‐dwelling and pathogenic bacteria is phosphorus limitation, an essential element for cellular processes. Here, we describe cellular and extracellular proteins differentially regulated between phosphate‐deplete (0 mM, no added phosphate) and phosphate‐replete (1 mM) growth conditions using a comparative proteomics (LC–MS/MS) approach. We observed a total of 128 and 65 unique proteins were downregulated and upregulated respectively, in the B. cenocepacia proteome. Of those downregulated proteins, many have functions in amino acid transport/metabolism. We have identified 24 upregulated proteins that are directly/indirectly involved in inorganic phosphate or organic phosphorus acquisition. Also, proteins involved in virulence and antimicrobial resistance were differentially regulated, suggesting B. cenocepacia experiences a dramatic shift in metabolism under these stress conditions. Overall, this study provides a baseline for further research into the biology of Burkholderia in response to phosphorus stress.
Item Type: | Journal Article | |||||||||
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Subjects: | Q Science > QD Chemistry Q Science > QH Natural history Q Science > QP Physiology |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | |||||||||
Library of Congress Subject Headings (LCSH): | Burkholderiales , Proteomics -- Research, Phosphorus | |||||||||
Journal or Publication Title: | Environmental Microbiology | |||||||||
Publisher: | Blackwell | |||||||||
ISSN: | 1462-2912 | |||||||||
Official Date: | September 2021 | |||||||||
Dates: |
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Volume: | 23 | |||||||||
Number: | 9 | |||||||||
Page Range: | pp. 5069-5086 | |||||||||
DOI: | 10.1111/1462-2920.15451 | |||||||||
Status: | Peer Reviewed | |||||||||
Publication Status: | Published | |||||||||
Access rights to Published version: | Open Access (Creative Commons) | |||||||||
Copyright Holders: | © 2021 The Authors. Environmental Microbiology published by Society for Applied Microbiology and John Wiley & Sons Ltd. | |||||||||
Date of first compliant deposit: | 4 March 2021 | |||||||||
Date of first compliant Open Access: | 23 March 2021 | |||||||||
RIOXX Funder/Project Grant: |
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