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Properties and mechanism of action of a 17 amino acid, V3 loop-specific microantibody that binds to and neutralizes human immunodeficiency virus type 1 virions
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UNSPECIFIED (1999) Properties and mechanism of action of a 17 amino acid, V3 loop-specific microantibody that binds to and neutralizes human immunodeficiency virus type 1 virions. JOURNAL OF GENERAL VIROLOGY, 80 (Part 1). pp. 225-236. ISSN 0022-1317.
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Abstract
Only two virus-neutralizing peptide microantibodies (MicroAbs) have been described and little is known about their mode of action. This report concerns a 17 amino acid cyclized MicroAb, derived from the third complementarity-determining region of the heavy chain of MAb F58 (IgG1), that recognizes the same minimum epitope in the V3 loop of the gp120 envelope protein of human immunodeficiency virus type 1 (HIV-1) as the MAb, The MicroAb was able to bind to and neutralize free virus particles. It was up to 5-fold more efficient in mass terms than F58 IgG and its neutralization rate on a molar basis was only 32-fold lower. The mechanism of neutralization of the MicroAb was also investigated. A high level of neutralization (99%) occurred without any significant decrease in attachment of virus to target C8166 cells. Neutralized virus attached to CD4, the HIV-1 primary receptor. Fusion of virions to cells was partially inhibited by the MicroAb, whereas F58 IgG has been shown to inhibit fusion significantly. Thus, neutralization by the MicroAb appears to be mediated, at least in part, by inhibition of fusion. Control peptides, in which the tyrosine at position 5 or 6 was deleted or changed to phenylalanine, showed no antiviral activity, attesting to the specificity of interaction of the MicroAb with the virion. It therefore appears that the MicroAb acts like an immunoglobulin. The data also show that the MicroAb/MAb F58 epitope on the V3 loop is not involved in attachment of virus to CD4 but is required for subsequent events in early infection.
| Item Type: | Journal Article | ||||
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| Subjects: | T Technology > TP Chemical technology Q Science > QR Microbiology > QR355 Virology |
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| Journal or Publication Title: | JOURNAL OF GENERAL VIROLOGY | ||||
| Publisher: | SOC GENERAL MICROBIOLOGY | ||||
| ISSN: | 0022-1317 | ||||
| Official Date: | January 1999 | ||||
| Dates: |
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| Volume: | 80 | ||||
| Number: | Part 1 | ||||
| Number of Pages: | 12 | ||||
| Page Range: | pp. 225-236 | ||||
| Publication Status: | Published |
Data sourced from Thomson Reuters' Web of Knowledge
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