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Collective dynein transport of the nucleus by pulling on astral microtubules during Saccharomyces cerevisiae mitosis
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Jain, Kunalika, Khetan, Neha, Yadav, Shivani A., Palani, Saravanan and Athale, Chaitanya A. (2021) Collective dynein transport of the nucleus by pulling on astral microtubules during Saccharomyces cerevisiae mitosis. Yeast, 38 (6). pp. 352-366. doi:10.1002/yea.3552 ISSN 0749-503X.
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Official URL: http://dx.doi.org/10.1002/yea.3552
Abstract
Positioning the nucleus at the bud neck during Saccharomyces cerevisiae mitosis involves pulling forces of cytoplasmic dynein localized in the daughter cell. Although genetic analysis has revealed a complex network positioning the nucleus, quantification of the forces acting on the nucleus and the number of dyneins driving the process has remained difficult. To better understand the collective forces involved in nuclear positioning, we compare a model of dyneins-driven microtubule (MT) pulling, MT pushing, and cytoplasmic drag to experiments. During S. cerevisiae mitosis, MTs interacting with the cortex nucleated by the daughter spindle pole body (SPB) (SPB-D) are longer than the mother SPB (SPB-M), increasing further during spindle elongation in anaphase. Interphasic SPB mobility is effectively diffusive, while the mitotic mobility is directed. By optimizing a computational model of the mobility of the nucleus due to diffusion and MTs pushing at the cell membrane to experiment, we estimate the viscosity governing the drag force on nuclei during positioning. A force balance model of mitotic SPB mobility compared to experimental mobility suggests that even one or two dynein dimers are sufficient to move the nucleus in the bud neck. Using stochastic computer simulations of a budding cell, we find that punctate dynein localization can generate sufficient force to reel in the nucleus to the bud neck. Compared to uniform motor localization, puncta involve fewer motors suggesting a functional role for motor clustering. Stochastic simulations also suggest that a higher number of force generators than predicted by force balance may be required to ensure the robustness of spindle positioning.
Item Type: | Journal Article | ||||||||
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Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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SWORD Depositor: | Library Publications Router | ||||||||
Journal or Publication Title: | Yeast | ||||||||
Publisher: | John Wiley & Sons Ltd. | ||||||||
ISSN: | 0749-503X | ||||||||
Official Date: | June 2021 | ||||||||
Dates: |
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Volume: | 38 | ||||||||
Number: | 6 | ||||||||
Page Range: | pp. 352-366 | ||||||||
DOI: | 10.1002/yea.3552 | ||||||||
Status: | Peer Reviewed | ||||||||
Publication Status: | Published | ||||||||
Access rights to Published version: | Restricted or Subscription Access | ||||||||
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