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Synedrella nodiflora extract depresses excitatory synaptic transmission and chemically-induced in vitro seizures in the rat hippocampus
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Amoateng, Patrick, Tagoe, Thomas A., Karikari, Thomas K., Kukuia, Kennedy K. E., Osei-Safo, Dorcas, Woode, Eric, Frenguelli, Bruno G. and Kombian, Samuel B. (2021) Synedrella nodiflora extract depresses excitatory synaptic transmission and chemically-induced in vitro seizures in the rat hippocampus. Frontiers in Pharmacology, 12 . 610025. doi:10.3389/fphar.2021.610025
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WRAP-Synedrella-nodiflora-depresses-excitatory-vitro-seizures-rat-hippocampus-2021.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (2306Kb) | Preview |
Official URL: http://dx.doi.org/10.3389/fphar.2021.610025
Abstract
Extracts of the tropical Cinderella plant Synedrella nodiflora are used traditionally to manage convulsive conditions in the West African sub-region. This study sought to determine the neuronal basis of the effectiveness of these plant extracts to suppress seizure activity. Using the hippocampal slice preparation from rats, the ability of the extract to depress excitatory synaptic transmission and in vitro seizure activity were investigated. Bath perfusion of the hydro-ethanolic extract of Synedrella nodiflora (SNE) caused a concentration-dependent depression of evoked field excitatory postsynaptic potentials (fEPSPs) recorded extracellularly in the CA1 region of the hippocampus with maximal depression of about 80% and an estimated IC50 of 0.06 mg/ml. The SNE-induced fEPSP depression was accompanied by an increase in paired pulse facilitation. The fEPSP depression only recovered partially after 20 min washing out. The effect of SNE was not stimulus dependent as it was present even in the absence of synaptic stimulation. Furthermore, it did not show desensitization as repeat application after 10 min washout produced the same level of fEPSP depression as the first application. The SNE effect on fEPSPs was not via adenosine release as it was neither blocked nor reversed by 8-CPT, an adenosine A1 receptor antagonist. In addition, SNE depressed in vitro seizures induced by zero Mg2+ and high K+ -containing artificial cerebrospinal fluid (aCSF) in a concentration-dependent manner. The results show that SNE depresses fEPSPs and spontaneous bursting activity in hippocampal neurons that may underlie its ability to abort convulsive activity in persons with epilepsy.
| Item Type: | Journal Article | ||||||
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| Subjects: | Q Science > QK Botany Q Science > QP Physiology R Medicine > RC Internal medicine R Medicine > RX Homeopathy |
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| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||
| Library of Congress Subject Headings (LCSH): | Compositae -- Utilization , Adenosine , Convulsions , Convulsions -- Homeopathic treatment -- West Africa, Hippocampus (Brain) , Epilepsy -- Animal models | ||||||
| Journal or Publication Title: | Frontiers in Pharmacology | ||||||
| Publisher: | Frontiers Research Foundation | ||||||
| ISSN: | 1663-9812 | ||||||
| Official Date: | 8 March 2021 | ||||||
| Dates: |
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| Volume: | 12 | ||||||
| Article Number: | 610025 | ||||||
| DOI: | 10.3389/fphar.2021.610025 | ||||||
| Status: | Peer Reviewed | ||||||
| Publication Status: | Published | ||||||
| Access rights to Published version: | Open Access | ||||||
| RIOXX Funder/Project Grant: |
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