Skip to content Skip to navigation
University of Warwick
  • Study
  • |
  • Research
  • |
  • Business
  • |
  • Alumni
  • |
  • News
  • |
  • About

University of Warwick
Publications service & WRAP

Highlight your research

  • WRAP
    • Home
    • Search WRAP
    • Browse by Warwick Author
    • Browse WRAP by Year
    • Browse WRAP by Subject
    • Browse WRAP by Department
    • Browse WRAP by Funder
    • Browse Theses by Department
  • Publications Service
    • Home
    • Search Publications Service
    • Browse by Warwick Author
    • Browse Publications service by Year
    • Browse Publications service by Subject
    • Browse Publications service by Department
    • Browse Publications service by Funder
  • Help & Advice
University of Warwick

The Library

  • Login
  • Admin

ARHGEF18/p114RhoGEF coordinates PKA/CREB signalling and actomyosin remodelling to promote trophoblast cell-cell fusion during placenta morphogenesis

Tools
- Tools
+ Tools

Beal, Robert, Alonso-Carriazo, Fernandez, Grammatopoulos, Dimitris, Matter, Karl and Balda, Maria S. (2021) ARHGEF18/p114RhoGEF coordinates PKA/CREB signalling and actomyosin remodelling to promote trophoblast cell-cell fusion during placenta morphogenesis. Frontiers in Cell and Developmental Biology, 9 . 658006. doi:10.3389/fcell.2021.658006

[img]
Preview
PDF
WRAP-signalling-actomyosin-remodelling-promote-trophoblast-cell-cell-fusion-during-placenta-morphogenesis-Grammatopoulos-2021.pdf - Published Version - Requires a PDF viewer.
Available under License Creative Commons Attribution 4.0.

Download (7Mb) | Preview
[img] PDF
ms-090321-wrap--authors_proof-3.pdf - Accepted Version
Embargoed item. Restricted access to Repository staff only - Requires a PDF viewer.

Download (8Mb)
Official URL: http://dx.doi.org/10.3389/fcell.2021.658006

Request Changes to record.

Abstract

Coordination of cell-cell adhesion, actomyosin dynamics and gene expression is crucial for morphogenetic processes underlying tissue and organ development. Rho GTPases are main regulators of the cytoskeleton and adhesion. They are activated by guanine nucleotide exchange factors in a spatially and temporally controlled manner. However, the roles of these Rho GTPase activators during complex developmental processes are still poorly understood. ARHGEF18/p114RhoGEF is a tight junction-associated RhoA activator that forms complexes with myosin II, and regulates actomyosin contractility. Here we show that p114RhoGEF/ARHGEF18 is required for mouse syncytiotrophoblast differentiation and placenta development. In vitro and in vivo experiments identify that p114RhoGEF controls expression of AKAP12, a protein regulating protein kinase A (PKA) signaling, and is required for PKA-induced actomyosin remodeling, cAMPresponsive element binding protein (CREB)-driven gene expression of proteins required for trophoblast differentiation, and, hence, trophoblast cell-cell fusion. Our data thus indicate that p114RhoGEF links actomyosin dynamics and cell-cell junctions to PKA/CREB signaling, gene expression and cell-cell fusion.

Item Type: Journal Article
Subjects: Q Science > QH Natural history
Q Science > QP Physiology
Divisions: Faculty of Medicine > Warwick Medical School > Biomedical Sciences
Faculty of Medicine > Warwick Medical School
Library of Congress Subject Headings (LCSH): Cell adhesion, Gene expression, Protein binding, G proteins, Rho GTPases
Journal or Publication Title: Frontiers in Cell and Developmental Biology
Publisher: Frontiers Media
ISSN: 2296-634X
Official Date: 25 March 2021
Dates:
DateEvent
25 March 2021Available
3 March 2021Accepted
Volume: 9
Article Number: 658006
DOI: 10.3389/fcell.2021.658006
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Open Access
RIOXX Funder/Project Grant:
Project/Grant IDRIOXX Funder NameFunder ID
BB/N001133/1 [BBSRC] Biotechnology and Biological Sciences Research Councilhttp://dx.doi.org/10.13039/501100000268
BB/N014855/1[BBSRC] Biotechnology and Biological Sciences Research Councilhttp://dx.doi.org/10.13039/501100000268
Related URLs:
  • Publisher

Request changes or add full text files to a record

Repository staff actions (login required)

View Item View Item
twitter

Email us: wrap@warwick.ac.uk
Contact Details
About Us