The Library
Proteomic analysis of drought & oxidative stress in Arabidopsis thaliana & Zea mays
Tools
Charlton, Georgina (2020) Proteomic analysis of drought & oxidative stress in Arabidopsis thaliana & Zea mays. PhD thesis, University of Warwick.
|
PDF
WRAP_Theses_Charlton_2020.pdf - Submitted Version - Requires a PDF viewer. Download (7Mb) | Preview |
Official URL: http://webcat.warwick.ac.uk/record=b3492967~S15
Abstract
Stress in plants can be caused by a variety of factors and create changes to proteins in the form of post translational modifications (PTMs). Reactive oxygen species (ROS) mediate cell signalling pathways but under stress conditions, their quantities become too high and they cause oxidative damage to DNA, lipids and proteins. Oxidative damage causes irreversible damage to proteins, leading to loss or change in function. Carbonylation modifications are used as key biomarkers of oxidative stress in animals and plants due to their relative chemical stability. However, due to their low abundance, carbonyl modifications have been difficult to analyse. Therefore, a suitable tagging and enrichment process is needed to fully analyse carbonyl modifications. In this thesis, the effect of drought stress on protein abundance in maize B73 was analysed. Using the tandem mass tag (TMT) for quantitative analysis 271 proteins were identified in the leaves and 405 proteins in the silks which changed significantly during drought stress. Phosphorylation as downstream signalling of drought stress was also analysed. 61 phosphopeptides were identified in the leaves and 130 phosphopeptides in the silks which significantly changed in the drought treated samples. These were novel phosphorylation sites. The effect of methyl viologen (MV), a herbicide which kills plants by causing oxidative damage, on protein abundance in Arabidopsis thaliana and maize B73 was also analysed. Using TMT for quantitative analysis 397 proteins were identified in the Arabidopsis soluble fraction and 578 proteins in the Arabidopsis membrane fraction with significantly changed abundance during MV treatment. MV’s effect on phosphorylation as downstream signalling was also analysed 84 phosphopeptides were identified in the Arabidopsis soluble fraction (five of which were novel phosphopeptides) and 42 phosphopeptides in the Arabidopsis membrane fraction (fourteen of which were novel phosphopeptides) which significantly changed in the methyl viologen treated samples. The effect of MV on maize was analysed, whilst there was no change in proteins identified using TMT quantification, 19 phosphopeptides were identified which significantly changed in the MV treatment, all of which were novel phosphopeptides. An attempt to enrich for carbonyl modifications in BSA was also made, for the purpose of enriching for carbonyl modifications in complex mixtures, however due to time constraints, this was not achievable.
Item Type: | Thesis (PhD) | ||||
---|---|---|---|---|---|
Alternative Title: | Proteomic analysis of drought and oxidative stress in Arabidopsis thaliana and Zea mays | ||||
Subjects: | Q Science > QD Chemistry Q Science > QH Natural history > QH426 Genetics Q Science > QK Botany |
||||
Library of Congress Subject Headings (LCSH): | Plant proteins, Post-translational modification, Corn -- Effect of stress on, Corn -- Effect of drought on, Arabidopsis thaliana -- Effect of stress on, Arabidopsis thaliana -- Effect of drought on, Oxidative stress | ||||
Official Date: | March 2020 | ||||
Dates: |
|
||||
Institution: | University of Warwick | ||||
Theses Department: | Department of Chemistry | ||||
Thesis Type: | PhD | ||||
Publication Status: | Unpublished | ||||
Supervisor(s)/Advisor: | Jones, Alexandra M. ; Kilby, Peter (Researcher in proteomics) ; Sinclair, John (Researcher in proteomics) | ||||
Sponsors: | Syngenta Seeds Ltd. ; Engineering and Physical Sciences Research Council ; University of Warwick. Molecular Analytical Science Centre for Doctoral Training | ||||
Format of File: | |||||
Extent: | xx, 250 leaves : illustrations (some colour) | ||||
Language: | eng |
Request changes or add full text files to a record
Repository staff actions (login required)
View Item |
Downloads
Downloads per month over past year