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Biotransformation of aromatic compounds by immobilized bacterial strains in barium alginate beads

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UNSPECIFIED (1999) Biotransformation of aromatic compounds by immobilized bacterial strains in barium alginate beads. ENZYME AND MICROBIAL TECHNOLOGY, 24 (3-4). pp. 232-236. ISSN 0141-0229

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Abstract

In the present study, we have investigated the biotransformation of different aromatic compounds to their cis-dihydrodiols (cis-diols) with immobilized Pseudomonas putida UV4 cells using barium alginate as the immobilization medium for the cells in a fluidized-bed reactor. The reactions have been performed in a biphasic system containing 20% (v/v) tetradecane. The bacterial beads were fluidized using a flow of pure oxygen at a flow rate of tao mi min(-1). The production of toluene cis-diol under the same conditions by the recombinant organism Escherichia coli JM109 (pKST11) was compared. The production of toluene cis-diol with E. coli JM109 (pKST11) was more than 24 times higher that the values reported in the literature using similar recombinant non-immobilized organisms. The capacity of immobilized E. coli JM109 (pKST11 to yield cis-diols from different aromatic substrates in small-scale biotransformations was also investigated. (C) 1998 Elsevier Science Inc.

Item Type: Journal Article
Subjects: T Technology > TP Chemical technology
Journal or Publication Title: ENZYME AND MICROBIAL TECHNOLOGY
Publisher: ELSEVIER SCIENCE INC
ISSN: 0141-0229
Date: February 1999
Volume: 24
Number: 3-4
Number of Pages: 5
Page Range: pp. 232-236
Publication Status: Published
URI: http://wrap.warwick.ac.uk/id/eprint/15005

Data sourced from Thomson Reuters' Web of Knowledge

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