Biotransformation of aromatic compounds by immobilized bacterial strains in barium alginate beads
UNSPECIFIED (1999) Biotransformation of aromatic compounds by immobilized bacterial strains in barium alginate beads. ENZYME AND MICROBIAL TECHNOLOGY, 24 (3-4). pp. 232-236. ISSN 0141-0229Full text not available from this repository.
In the present study, we have investigated the biotransformation of different aromatic compounds to their cis-dihydrodiols (cis-diols) with immobilized Pseudomonas putida UV4 cells using barium alginate as the immobilization medium for the cells in a fluidized-bed reactor. The reactions have been performed in a biphasic system containing 20% (v/v) tetradecane. The bacterial beads were fluidized using a flow of pure oxygen at a flow rate of tao mi min(-1). The production of toluene cis-diol under the same conditions by the recombinant organism Escherichia coli JM109 (pKST11) was compared. The production of toluene cis-diol with E. coli JM109 (pKST11) was more than 24 times higher that the values reported in the literature using similar recombinant non-immobilized organisms. The capacity of immobilized E. coli JM109 (pKST11 to yield cis-diols from different aromatic substrates in small-scale biotransformations was also investigated. (C) 1998 Elsevier Science Inc.
|Item Type:||Journal Article|
|Subjects:||T Technology > TP Chemical technology|
|Journal or Publication Title:||ENZYME AND MICROBIAL TECHNOLOGY|
|Publisher:||ELSEVIER SCIENCE INC|
|Number of Pages:||5|
|Page Range:||pp. 232-236|
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