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Biotransformation of aromatic compounds by immobilized bacterial strains in barium alginate beads
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UNSPECIFIED (1999) Biotransformation of aromatic compounds by immobilized bacterial strains in barium alginate beads. ENZYME AND MICROBIAL TECHNOLOGY, 24 (3-4). pp. 232-236. ISSN 0141-0229.
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Abstract
In the present study, we have investigated the biotransformation of different aromatic compounds to their cis-dihydrodiols (cis-diols) with immobilized Pseudomonas putida UV4 cells using barium alginate as the immobilization medium for the cells in a fluidized-bed reactor. The reactions have been performed in a biphasic system containing 20% (v/v) tetradecane. The bacterial beads were fluidized using a flow of pure oxygen at a flow rate of tao mi min(-1). The production of toluene cis-diol under the same conditions by the recombinant organism Escherichia coli JM109 (pKST11) was compared. The production of toluene cis-diol with E. coli JM109 (pKST11) was more than 24 times higher that the values reported in the literature using similar recombinant non-immobilized organisms. The capacity of immobilized E. coli JM109 (pKST11 to yield cis-diols from different aromatic substrates in small-scale biotransformations was also investigated. (C) 1998 Elsevier Science Inc.
Item Type: | Journal Article | ||||
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Subjects: | T Technology > TP Chemical technology | ||||
Journal or Publication Title: | ENZYME AND MICROBIAL TECHNOLOGY | ||||
Publisher: | ELSEVIER SCIENCE INC | ||||
ISSN: | 0141-0229 | ||||
Official Date: | February 1999 | ||||
Dates: |
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Volume: | 24 | ||||
Number: | 3-4 | ||||
Number of Pages: | 5 | ||||
Page Range: | pp. 232-236 | ||||
Publication Status: | Published |
Data sourced from Thomson Reuters' Web of Knowledge
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