Kikuchi-Taura, Akie, Okinaka, Yuka, Saino, Orie, Takeuchi, Yukiko, Ogawa, Yuko, Kimura, Takafumi, Gul, Sheraz, Claussen, Carsten, Boltze, Johannes and Taguchi, Akihiko (2021) Gap junction-mediated cell-cell interaction between transplanted mesenchymal stem cells and vascular endothelium in stroke. Stem Cells, 39 (7). pp. 904-912. doi:10.1002/stem.3360 ISSN 1066-5099.

Preview	PDF WRAP-Gap-junction-cell-transplanted-mesenchymal-stem-cells-vascular-endothelium-stroke-2021.pdf - Accepted Version - Requires a PDF viewer. Download (1137Kb) | Preview
Preview	PDF WRAP-Graphical-abstract-2021.pdf - Supplemental Material - Requires a PDF viewer. Download (360Kb) | Preview
	PDF WRAP-Figure-2021.pptx - Supplemental Material - Requires a PDF viewer. Download (1336Kb)

Request Changes to record.

Abstract

We have shown previously that transplanted bone marrow mononuclear cells (BM‐MNC), which are a cell fraction rich in hematopoietic stem cells, can activate cerebral endothelial cells via gap junction‐mediated cell‐cell interaction. In the present study, we investigated such cell‐cell interaction between mesenchymal stem cells (MSC) and cerebral endothelial cells. In contrast to BM‐MNC, for MSC we observed suppression of vascular endothelial growth factor uptake into endothelial cells and transfer of glucose from endothelial cells to MSC in vitro. The transfer of such a small molecule from MSC to vascular endothelium was subsequently confirmed in vivo and was followed by suppressed activation of macrophage/microglia in stroke mice. The suppressive effect was absent by blockade of gap junction at MSC. Furthermore, gap junction‐mediated cell‐cell interaction was observed between circulating white blood cells and MSC. Our findings indicate that gap junction‐mediated cell‐cell interaction is one of the major pathways for MSC‐mediated suppression of inflammation in the brain following stroke and provides a novel strategy to maintain the blood‐brain barrier in injured brain. Furthermore, our current results have the potential to provide a novel insight for other ongoing clinical trials that make use of MSC transplantation aiming to suppress excess inflammation, as well as other diseases such as COVID‐19 (coronavirus disease 2019).

Item Type:	Journal Article
Subjects:	Q Science > QH Natural history > QH426 Genetics Q Science > QP Physiology
Divisions:	Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- )
SWORD Depositor:	Library Publications Router
Library of Congress Subject Headings (LCSH):	Mesenchymal stem cells, Stem cells -- Research, Vascular endothelium, Cerebrovascular disease, Gap junctions (Cell biology), Immunosuppression
Journal or Publication Title:	Stem Cells
Publisher:	AlphaMed Press, Inc.
ISSN:	1066-5099
Official Date:	July 2021
Dates:	Date Event July 2021 Published 11 March 2021 Available 5 February 2021 Accepted
Volume:	39
Number:	7
Page Range:	pp. 904-912
DOI:	10.1002/stem.3360
Status:	Peer Reviewed
Publication Status:	Published
Reuse Statement (publisher, data, author rights):	"This is the peer reviewed version of the following article: Kikuchi‐Taura, A, Okinaka, Y, Saino, O, et al. Gap junction‐mediated cell‐cell interaction between transplanted mesenchymal stem cells and vascular endothelium in stroke. Stem Cells. 2021; 1– 9, which has been published in final form at https://doi.org/10.1002/stem.3360. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions."
Access rights to Published version:	Open Access (Creative Commons)
Date of first compliant deposit:	5 May 2021
Date of first compliant Open Access:	11 September 2021
RIOXX Funder/Project Grant:	Project/Grant ID RIOXX Funder Name Funder ID A79 Japan Agency for Medical Research and Development http://dx.doi.org/10.13039/100009619
Is Part Of:	1

Request changes or add full text files to a record

Repository staff actions (login required)

View Item

Downloads